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Cytokeratin 14 Antibody (RCK107): sc-23878

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Datasheets
  • Cytokeratin 14 Antibody (RCK107) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
  • raised against Cytoskeletal preparation of TR146 epithelial cells
  • recommended for detection of Cytokeratin 14 of mouse, rat, human, porcine and canine origin by WB and IF
  • See Cytokeratin 14 (LL001): sc-53253 for Cytokeratin 14 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cytokeratin 14 Antibody (RCK107) for WB applications. These reagents are now offered in bundles with Cytokeratin 14 Antibody (RCK107) (see ordering information below).

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Cytokeratin 14 Antibody (RCK107) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 14 in mouse, rat, human, porcine, and canine samples through applications such as western blotting (WB) and immunofluorescence (IF). Cytokeratin 14 is a vital component of the cytoskeletal structure in epithelial cells, primarily found in the basal layer of the epidermis, where Cytokeratin 14 plays a crucial role in maintaining cellular integrity and resilience against mechanical stress. Cytokeratin 14 forms heterodimers with other cytokeratins, contributing to the formation of intermediate filaments that provide structural support to keratinocytes. Proper functioning of Cytokeratin 14 is essential for skin health, as mutations in the gene encoding Cytokeratin 14 can lead to epidermolysis bullosa simplex, a genetic disorder characterized by fragile skin that blisters easily. Expression of Cytokeratin 14 is also significant in various skin diseases, including Bowen′s disease, where distinct expression patterns can aid in diagnosis and characterization of malignant tumors. Anti-Cytokeratin 14 antibody (RCK107) serves as an invaluable tool for researchers studying epithelial cell biology, skin disorders, and cancer pathology, allowing detailed analysis in various species.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cytokeratin 14 Antibody (RCK107)

sc-23878
200 µg/ml
$316.00

Cytokeratin 14 Antibody (RCK107): m-IgG Fc BP-HRP Bundle

sc-536682
200 µg Ab; 10 µg BP
$354.00

Cytokeratin 14 Antibody (RCK107): m-IgGκ BP-HRP Bundle

sc-533841
200 µg Ab; 40 µg BP
$354.00

Cytokeratin 14 Antibody (RCK107): m-IgG1 BP-HRP Bundle

sc-544762
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with Cytokeratin 14 (RCK107): sc-23878 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well in IF publication with rat esophagealWorks well in IF publication with rat esophageal epithelial samples. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in HeLa samples. -SCBT QC
Date published: 2015-02-07
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Cytokeratin 14 Antibody (RCK107) is rated 5.0 out of 5 by 2.
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