Cytokeratin 14 Antibody (LL001) recommended for detection of Cytokeratin 14 of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)

Cytokeratin 14 Antibody (LL001): sc-53253

Cytokeratin 14 Antibody (LL001) is rated 4.3 out of 5 by 19.
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Datasheets

    • Cytokeratin 14 Antibody (LL001) is a mouse monoclonal IgG2a κ, cited in 91 publications, provided at 200 µg/ml
    • raised against peptide (last 16 carboxy-terminal amino acids of human keratin) coupled to thyroglobulin by the extra Cys added at the N terminus
    • Anti-Cytokeratin 14 Antibody (LL001) is recommended for detection of Cytokeratin 14 of mouse, rat, human and porcine origin by WB, IP, IF and IHC(P)
    • Anti-Cytokeratin 14 Antibody (LL001) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 14 (LL001): sc-53253.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Cytokeratin 14 Antibody (LL001) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 14 Antibody (LL001) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Is it possible to use the HRP conjugated form sc-53253 HRP in both Western Blot and IP?

Asked by: AbPolly
Thank you for your inquiry. We do not recommend the use of the HRP conjugated form in IP. The unconjugated form of the antibody is the best choice when working in multiple applications that do not use the same conjugation (Agarose, Biotin, HRP, FITC, etc.).
Answered by: Technical Support 12
Date published: 2017-01-04

Is this antibody suitable for Flow cytometry?

Asked by: Emomed
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 1 out of 5 by from Not working on paraffin embedded tissue Tried different retrieval buffers (pH6, pH9), blocking with BSA/FCS, no specific signal detected with direct conjugated AF647.
Date published: 2019-08-26
Rated 1 out of 5 by from No signal Used on FFPE mammary glands, IF staining, no signal.
Date published: 2018-07-06
Rated 5 out of 5 by from Excellent. I bought this product a month ago and it is really working well.
Date published: 2018-06-20
Rated 3 out of 5 by from Specific but low signal Detection of basal breast ductal cells is specific but poor at 1/50
Date published: 2018-05-12
Rated 5 out of 5 by from Great for IHC staining. I got the free sample one month ago, and this antibody works very well for IHC staining.
Date published: 2017-10-11
Rated 4 out of 5 by from this antibody i used this antibody for IHC-P on chicken skin section,but no positive signals were found
Date published: 2017-09-07
Rated 5 out of 5 by from Great antibody I got the antibody one month ago and it worked well.
Date published: 2017-03-22
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