Cytokeratin 13 Antibody (AE8) recommended for detection of Cytokeratin 13 of mouse, rat, human and rabbit origin by WB, IP, IF and IHC(P)

Cytokeratin 13 Antibody (AE8): sc-57003

Cytokeratin 13 Antibody (AE8) is rated 5.0 out of 5 by 3.
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Datasheets

    • Cytokeratin 13 Antibody (AE8) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against esophageal keratins of rabbit origin
    • recommended for detection of Cytokeratin 13 of mouse, rat, human and rabbit origin by WB, IP, IF and IHC(P)
    • See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 13 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using Cytokeratin 13 (AE8): sc-57003 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great monoclonal antibody Cytokeratin 13 Antibody (AE8) showed strong band with very little background and nice IHC stain
Date published: 2017-02-07
Rated 5 out of 5 by from Good nuclear staining with paraffin Good nuclear staining with paraffin-embedded human esophagus samples. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Works well for WB with human throat tissue Works well for WB with human throat tissue extract and A-431 whole cell lysate. -SCBT QC
Date published: 2013-01-19
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