Cytokeratin 1 Antibody (E-12): sc-376224
- Cytokeratin 1 Antibody (E-12) is a mouse monoclonal IgG2a κ Cytokeratin 1 antibody, cited in 12 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 209-247 within an internal region of Cytokeratin 1 of human origin
- Cytokeratin 1 Antibody (E-12) is recommended for detection of Cytokeratin 1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cytokeratin 1 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cytokeratin 1 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 1 Antibody (E-12) (see ordering information below).
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Cytokeratin 1 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cytokeratin 1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Cytokeratin 1 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 1, also known as KRT1, is a vital component of the cytoskeletal structure in epithelial cells, playing a crucial role in maintaining cellular integrity and resilience against mechanical stress. KRT1 is primarily located in the cytoplasm of keratinocytes, forming part of the intermediate filament network that provides structural support and stability to cells. KRT1 expression is particularly important in the epidermis, contributing to skin barrier formation, which protects against environmental damage and prevents water loss. Additionally, KRT1 serves as a key marker for various epithelial malignancies, including epithelioid hemangioendotheliomas and angiosarcomas, making anti-KRT1 antibody (E-12) a valuable tool for cancer diagnosis and research. The gene encoding human KRT1 is located on chromosome 12q13.13, and mutations in this gene can lead to abnormal filament formation and conditions such as epidermolytic hyperkeratosis, highlighting KRT1′s significance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cytokeratin 1 Antibody (E-12) References:
- Keratin 1 expression in endothelia and mesenchymal tumors: an immunohistochemical analysis of normal and neoplastic tissues. | Remotti, F., et al. 2001. Hum Pathol. 32: 873-9. PMID: 11521233
- Two type II keratin genes are localized on human chromosome 12. | Popescu, NC., et al. 1989. Hum Genet. 82: 109-12. PMID: 2470667
- Mutations in the H1 and 1A domains in the keratin 1 gene in epidermolytic hyperkeratosis. | Yang, JM., et al. 1994. J Invest Dermatol. 102: 17-23. PMID: 7507151
- Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview. | van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
- Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients. | Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
- Keratins and the skin. | Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
- Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis. | Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
- Cytokeratin expression, fibrillar organization, and subtle function in liver cells. | Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 1 Antibody (E-12) | sc-376224 | 200 µg/ml | $322.00 | |||
Cytokeratin 1 Antibody (E-12): m-IgG Fc BP-HRP Bundle | sc-529839 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cytokeratin 1 Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-522881 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cytokeratin 1 Antibody (E-12): m-IgG2a BP-HRP Bundle | sc-547390 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cytokeratin 1 Antibody (E-12) AC | sc-376224 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cytokeratin 1 Antibody (E-12) HRP | sc-376224 HRP | 200 µg/ml | $322.00 | |||
Cytokeratin 1 Antibody (E-12) FITC | sc-376224 FITC | 200 µg/ml | $336.00 | |||
Cytokeratin 1 Antibody (E-12) PE | sc-376224 PE | 200 µg/ml | $349.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 488 | sc-376224 AF488 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 546 | sc-376224 AF546 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 594 | sc-376224 AF594 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 647 | sc-376224 AF647 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 680 | sc-376224 AF680 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 Antibody (E-12) Alexa Fluor® 790 | sc-376224 AF790 | 200 µg/ml | $364.00 | |||
Cytokeratin 1 (E-12) Neutralizing Peptide | sc-376224 P | 100 µg/0.5 ml | $69.00 |