Cytokeratin 1/10 Antibody (LH1)recommended for detection of Cytokeratin 1 and Cytokeratin 10 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA

Cytokeratin 1/10 Antibody (LH1): sc-53251

Cytokeratin 1/10 Antibody (LH1) is rated 4.6 out of 5 by 5.
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Datasheets

    • Cytokeratin 1/10 Antibody (LH1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length proteins Cytokeratin 1 and 10 of human origin
    • Cytokeratin 1/10 Antibody (LH1) is recommended for detection of Cytokeratin 1 and Cytokeratin 10 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA
    • Cytokeratin 1/10 Antibody (LH1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 1/10 (LH1): sc-53251.
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I am using Cytokeratin 1/10 (LH1): sc-53251 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from positive in human keratinocytes positive result in keratinocytes with 1:200 dilution, recommended. santa cruz gives free samples and its overnight shipping.
Date published: 2017-11-14
Rated 5 out of 5 by from Positive results Cytokeratin 1/10 Antibody (LH1) produced robust IHCP stain and positive results by Western blot
Date published: 2017-01-27
Rated 4 out of 5 by from Positive WB data in Caco Positive WB data in Caco-2 whole cell lysate. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from WB publication in human squamous cell carcinoma WB publication in human squamous cell carcinoma. -SCBT Publication Review
Date published: 2015-01-31
Rated 4 out of 5 by from Great IHC data with paraffin Great IHC data with paraffin-embedded human vulva/anal skin tissue. -SCBT QC
Date published: 2015-01-04
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