CYP7A1 Antibody (8F1): sc-293193

CYP7A1 Antibody (8F1) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYP7A1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CYP7A1 antibody (8F1) is available as a non-conjugated format. CYP7A1, also known as cholesterol 7-alpha-hydroxylase, plays a crucial role as the rate-limiting enzyme in bile acid synthesis within the liver, a process essential for the digestion and absorption of dietary fats and fat-soluble vitamins. CYP7A1 expression is primarily mediated by the bile acid receptor FXR, which responds to the levels of bile acids in the body, ensuring a balance in bile acid homeostasis. Dysregulation of CYP7A1 can lead to metabolic disorders, including cholesterol gallstone disease and liver dysfunction. Additionally, CYP7A1 activity is influenced by various factors, including dietary components and hormonal signals, highlighting its importance in maintaining lipid metabolism and overall health. Anti-CYP7A1 antibody (8F1) serves as an invaluable tool for researchers studying cholesterol metabolism and related diseases, providing insights into the functional dynamics of CYP7A1 in human physiology.

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CYP7A1 Antibody (8F1) References:

1. The N-terminal LIM domain negatively regulates the kinase activity of LIM-kinase 1.  |  Nagata, K., et al. 1999. Biochem J. 343 Pt 1: 99-105. PMID: 10493917
2. Genetic variants of CYP19 (aromatase) and breast cancer risk.  |  Kristensen, VN., et al. 2000. Oncogene. 19: 1329-33. PMID: 10713674
3. Aromatase and gynecomastia.  |  Braunstein, GD. 1999. Endocr Relat Cancer. 6: 315-24. PMID: 10731125
4. Metabolism of vitamin D(3) by human CYP27A1.  |  Sawada, N., et al. 2000. Biochem Biophys Res Commun. 273: 977-84. PMID: 10891358
5. Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers.  |  Repa, JJ., et al. 2000. Science. 289: 1524-9. PMID: 10968783
6. Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM kinase.  |  Horita, Y., et al. 2008. J Biol Chem. 283: 6013-21. PMID: 18171679
7. LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats.  |  Li, Y., et al. 2014. Sci Signal. 7: ra61. PMID: 24962708
8. Combined LIM kinase 1 and p21-Activated kinase 4 inhibitor treatment exhibits potent preclinical antitumor efficacy in breast cancer.  |  Zhao, CC., et al. 2020. Cancer Lett. 493: 120-127. PMID: 32829006
9. NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.  |  Zhou, Z., et al. 2021. Cell Rep. 36: 109515. PMID: 34407403
10. P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature.  |  Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
11. P450BM-3; a tale of two domains--or is it three?  |  Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
12. Endocrine disorders associated with inappropriately high aromatase expression.  |  Bulun, SE., et al. 1997. J Steroid Biochem Mol Biol. 61: 133-9. PMID: 9365182

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CYP7A1 Antibody (8F1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CYP7A1 Antibody (8F1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CYP7A1 Antibody (8F1) Specifications

Alternate Product Name: Cytochrome P450 7A1

Product Clone Name: 8F1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 179-277

Epitope Target: CYP7A1

Epitope Species: human

Product Reactivity/Detection: CYP7A1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CYP7A1 Antibody (8F1): N/A

The preferred CYP7A1 antibody is CYP7A1 Antibody (E-10): sc-518007.