CUL-5 Antibody (F-6): sc-373822

CUL-5 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects CUL-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CUL-5 (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CUL-5 protein, also known as vasopressin-activated calcium-mobilizing protein (VACM-1), plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. Specifically, CUL-5 is involved in the formation of E3 ubiquitin ligase complexes, which facilitate the selective ubiquitination of target proteins, thereby influencing various cellular processes such as cell cycle progression and signal transduction. Proper functioning of CUL-5 is vital, as dysregulation of ubiquitin-mediated proteolysis can lead to various diseases, including cancer and neurodegenerative disorders. Using anti-CUL-5 antibody (F-6) in research can provide valuable insights into the mechanisms of protein regulation and CUL-5′s implications in health and disease.

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CUL-5 Antibody (F-6) References:

1. One ring to rule a superfamily of E3 ubiquitin ligases.  |  Tyers, M. and Willems, AR. 1999. Science. 284: 601, 603-4. PMID: 10328744
2. Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.  |  Singer, JD., et al. 1999. Genes Dev. 13: 2375-87. PMID: 10500095
3. Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex.  |  Iwai, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 12436-41. PMID: 10535940
4. Cullin-5 plays multiple roles in cell fate specification and synapse formation during Drosophila development.  |  Ayyub, C., et al. 2005. Dev Dyn. 232: 865-75. PMID: 15712282
5. Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI).  |  Yao, XL., et al. 2006. Neurosci Lett. 409: 65-9. PMID: 17010517
6. Reduced cul-5 activity causes aberrant follicular morphogenesis and germ cell loss in Drosophila oogenesis.  |  Kugler, JM., et al. 2010. PLoS One. 5: e9048. PMID: 20140218
7. Cutting Edge: A Cullin-5-TRAF6 Interaction Promotes TRAF6 Polyubiquitination and Lipopolysaccharide Signaling.  |  Zhu, Z., et al. 2016. J Immunol. 197: 21-6. PMID: 27233966
8. Cullin 5 aggravates hypoxic pulmonary hypertension by activating TRAF6/NF-κB/HIF-1α/VEGF.  |  Wang, L., et al. 2023. iScience. 26: 108199. PMID: 37965157
9. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.  |  Kipreos, ET., et al. 1996. Cell. 85: 829-39. PMID: 8681378
10. Identification and analysis of expression of human VACM-1, a cullin gene family member located on chromosome 11q22-23.  |  Byrd, PJ., et al. 1997. Genome Res. 7: 71-5. PMID: 9037604
11. The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers.  |  Chen, LC., et al. 1998. Cancer Res. 58: 3677-83. PMID: 9721878
12. Human CUL-1 associates with the SKP1/SKP2 complex and regulates p21(CIP1/WAF1) and cyclin D proteins.  |  Yu, ZK., et al. 1998. Proc Natl Acad Sci U S A. 95: 11324-9. PMID: 9736735

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUL-5 Antibody (F-6) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-CUL-5 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUL-5 Antibody (F-6) Specifications

Alternate Product Name: CUL-5

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: CUL-5 (cullin-5)

Epitope Species: human

Product Reactivity/Detection: CUL-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for CUL-5 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin