CUL-5 Antibody (F-6) recommended for detection of CUL-5 of mouse, rat and human origin by WB, IP, IF and ELISA

CUL-5 Antibody (F-6): sc-373822

CUL-5 Antibody (F-6) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-CUL-5 Antibody (F-6) is a mouse monoclonal IgG1 CUL-5 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of CUL-5 (cullin-5) of human origin
    • Anti-CUL-5 Antibody (F-6) is recommended for detection of CUL-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, bovine and porcine
    • Anti-CUL-5 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CUL-5 (F-6): sc-373822.
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What blocking solution should I use with CUL-5 (F-6): sc-373822 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 1 out of 5 by from This antibody does not work all that well I boght this antibody as my previous experience with SCBT has been good. But unfortuntely this antibody very faint band at 1:25 dilution.
Date published: 2019-12-02
Rated 5 out of 5 by from review for Cul-5 antibody This antibody works well on our hands, we get the results as predicted.
Date published: 2018-07-10
Rated 5 out of 5 by from 条带单一,特异性好 值得购买,WB效果非常不错。。。值得购买,WB效果非常不错。。。值得购买,WB效果非常不错。。。值得购买,WB效果非常不错。。。值得购买,WB效果非常不错。。。
Date published: 2018-01-27
Rated 5 out of 5 by from Good for WB It is a good antibody for Western blot with human cell nuclear extracts.
Date published: 2017-09-23
Rated 5 out of 5 by from Specific antibody for cullin-5 This is a great choice if you're looking for a very strong CUL-5 antibody, especially for WB. Nice solid bands every time!
Date published: 2017-01-16
Rated 5 out of 5 by from Produced nice Western blot data of CUL Produced nice Western blot data of CUL-5 expression in HeLa, SKBR-3, T-47D and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2013-04-18
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