CUL-2 Antibody (C-4) recommended for detection of CUL-2 of mouse, rat and human origin by WB, IP, IF and ELISA

CUL-2 Antibody (C-4): sc-166506

CUL-2 Antibody (C-4) is rated 4.8 out of 5 by 6.
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Datasheets

    • Anti-CUL-2 Antibody (C-4) is a mouse monoclonal IgG1 CUL-2 antibody provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of CUL-2 (cullin-2) of human origin
    • Anti-CUL-2 Antibody (C-4) is recommended for detection of CUL-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-CUL-2 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CUL-2 (C-4): sc-166506.
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What is the recommended fixation method for immunofluorescence staining with CUL-2 (C-4): sc-166506 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works well for WB This antibody worked well for WB and produced clean and good bands (1:200 in 5% milk).
Date published: 2018-10-16
Rated 4 out of 5 by from Works for WB This antibody works for Western blot with human cell nuclear extract.
Date published: 2017-09-23
Rated 5 out of 5 by from Published for Western blot This CUL-2 Antibody (C-4): sc-166506 has been featured in 4 publications for Western blot, including 2 within the last year!
Date published: 2017-02-02
Rated 5 out of 5 by from Robust signal in WB in K Robust signal in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Good specificity in WB in NIH/3T3 whole cell Good specificity in WB in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-17
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