CUL-1 Antibody (AS97) recommended for detection of CUL-1 of mouse, rat and human origin by WB and IP

CUL-1 Antibody (AS97): sc-12761

CUL-1 Antibody (AS97) is rated 4.2 out of 5 by 5.
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Datasheets

    • CUL-1 Antibody (AS97) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against recombinant Cul-1 of human origin
    • CUL-1 Antibody (AS97) is recommended for detection of CUL-1 of mouse, rat and human origin by WB and IP
    • CUL-1 Antibody (AS97) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CUL-1 (AS97): sc-12761.
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Can CUL-1 (AS97): sc-12761 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CUL-1 (AS97): sc-12761 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Great WB signal achieved in JAR whole cell lysate Great WB signal achieved in JAR whole cell lysate. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by from Nice publishable WB data on HEK293 cells Nice publishable WB data on HEK293 cells. -SCBT Publication Review
Date published: 2015-05-16
Rated 4 out of 5 by from Nice band acquired in WB using mouse CUL Nice band acquired in WB using mouse CUL-1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with human MMRU and PMWK. -SCBT Publication Review
Date published: 2015-01-09
Rated 5 out of 5 by from Published data for WB and IP in MDA Published data for WB and IP in MDA-MB-231, MCF-7 and SK-UT-113. -SCBT Publication Review
Date published: 2014-05-21
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