Date published: 2025-9-5
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CTLA-4 Antibody (F-8) - Western Blotting - Image 391449
CTLA-4 抗体 (F-8) Alexa Fluor® 488: sc-376016 AF488. 对 ALL-SIL (A) 和 CCRF-CEM (B) 全细胞裂解液中 CTLA-4 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
CTLA-4 抗体 (F-8) Alexa Fluor® 488: sc-376016 AF488. 对 ALL-SIL (A) 和 CCRF-CEM (B) 全细胞裂解液中 CTLA-4 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.

CTLA-4 抗体 (F-8): sc-376016

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说明书
  • CTLA-4 抗体 F-8 是小鼠单克隆 IgG1 κ，CTLA-4抗体, 在60篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CTLA-4的C-terminus的氨基酸195-223之间
  • CTLA-4 抗体 (F-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CTLA-4; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗CTLA-4抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CTLA-4 (F-8): sc-376016的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CTLA-4 抗体 (F-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CTLA-4 抗体 (F-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CTLA 抗体包括 CTLA-4

CTLA-4抗体（F-8）是一种IgG1 κ小鼠单克隆CTLA-4抗体（也称为CD152抗体或CTLA4抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的CTLA-4蛋白。CTLA-4抗体（F-8）既可以是非偶联形式的抗CTLA-4抗体，也可以是多种偶联形式的抗CTLA-4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。T细胞增殖和淋巴因子产生是由抗原占据TCR后触发的，随后由抗原呈递细胞上表达的配体传递共刺激信号。B7相关细胞表面蛋白CD80（B7-1）和CD86（B7-2）在抗原呈递细胞上表达，结合同源的T细胞受体CD28和CTLA-4（细胞毒性T淋巴细胞相关蛋白-4），并触发最佳的T细胞激活共刺激信号。CTLA-4与CD28具有31%的总体氨基酸同一性，因此有人提出CD28和CTLA-4在功能上是冗余的。SLAM是T细胞上的一种新型受体，当被激活时，它以不依赖CD28的方式增强T细胞扩增。B7，也称为BB1，是CD28和CTLA-4的另一种配体或对应受体，表达于抗原呈递细胞上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CTLA-4 抗体 (F-8) 参考文献:

  1. T 淋巴细胞的成本刺激：CD28, CTLA-4 和 B7/BB1 在白细胞介素-2 产物和免疫疗法中的作用。  |  Schwartz, RH. 1992. Cell. 71: 1065-8. PMID: 1335362
  2. 人类 B 淋巴细胞活化抗原 B7 的鼠同源物的结构, 表达和 T 细胞激动活性。  |  Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
  3. CTLA-4 对 CD8+ T 细胞上 CD28 表达的调控。  |  Berg, M. and Zavazava, N. 2008. J Leukoc Biol. 83: 853-63. PMID: 18162511
  4. DNA拓扑异构酶IIα通过转录激活β-catenin来促进胶质瘤细胞的转移特性。  |  Snyder, A., et al. 2014. N Engl J Med. 371: 2189-2199. PMID: 25409260
  5. 日本系统性硬化症患者体内抗DNA拓扑异构酶IIα自身抗体。  |  Verma, N., et al. 2017. Clin Exp Immunol. 190: 1-7. PMID: 28600865
  6. DNA拓扑异构酶II-α的表达：喉癌的临床意义。  |  Zhou, X., et al. 2023. Nat Immunol. 24: 1499-1510. PMID: 37500885
  7. 人类DNA拓扑异构酶II-α。  |  Ahmed, A., et al. 2024. Nature. 630: 976-983. PMID: 38867048
  8. B7 和 CD28 受体家族在自身免疫和免疫逃避中的潜在作用。  |  Harlan, DM., et al. 1995. Clin Immunol Immunopathol. 75: 99-111. PMID: 7535672
  9. 鉴定与 CD28 和 CTLA4 的功能性相互作用有关的 CD80 (B7-1) V 结构域残基。  |  Fargeas, CA., et al. 1995. J Exp Med. 182: 667-75. PMID: 7544393
  10. CD80 的两个细胞外免疫球蛋白样结构域都含有对结合 T 细胞表面受体 CTLA-4 和 CD28 至关重要的残基。  |  Peach, RJ., et al. 1995. J Biol Chem. 270: 21181-7. PMID: 7545666
  11. 一种参与 T 细胞活化的新型受体。  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
  12. CTLA4 介导人类 T 细胞的抗原特异性凋亡。  |  Gribben, JG., et al. 1995. Proc Natl Acad Sci U S A. 92: 811-5. PMID: 7846057

订购信息

产品名称产品编号规格价格数量收藏夹

CTLA-4 抗体 (F-8)

sc-376016
200 µg/ml
$316.00

CTLA-4 (F-8): m-IgG Fc BP-HRP 套装

sc-529801
200 µg Ab; 10 µg BP
$354.00

CTLA-4 (F-8): m-IgGκ BP-HRP 套装

sc-522818
200 µg Ab, 40 µg BP
$354.00

CTLA-4 (F-8): m-IgG1 BP-HRP 套装

sc-543694
200 µg Ab; 20 µg BP
$354.00

CTLA-4 抗体 (F-8) AC

sc-376016 AC
500 µg/ml, 25% agarose
$416.00

CTLA-4 抗体 (F-8) HRP

sc-376016 HRP
200 µg/ml
$316.00

CTLA-4 抗体 (F-8) FITC

sc-376016 FITC
200 µg/ml
$330.00

CTLA-4 抗体 (F-8) PE

sc-376016 PE
200 µg/ml
$343.00

CTLA-4 抗体 (F-8) Alexa Fluor® 488

sc-376016 AF488
200 µg/ml
$357.00

CTLA-4 抗体 (F-8) Alexa Fluor® 546

sc-376016 AF546
200 µg/ml
$357.00

CTLA-4 抗体 (F-8) Alexa Fluor® 594

sc-376016 AF594
200 µg/ml
$357.00

CTLA-4 抗体 (F-8) Alexa Fluor® 647

sc-376016 AF647
200 µg/ml
$357.00

CTLA-4 抗体 (F-8) Alexa Fluor® 680

sc-376016 AF680
200 µg/ml
$357.00

CTLA-4 抗体 (F-8) Alexa Fluor® 790

sc-376016 AF790
200 µg/ml
$357.00

CTLA-4 (F-8) 中和勝肽

sc-376016 P
100 µg/0.5 ml
$68.00

What is the specific region where this antibody is interacting? The datasheet says that the region of the interaction is between amino acids 195-223, but this is bigger region than an epitope region (6-8 aa). Is it recognizes a discontinuous epitope?

Asked by: Marysol
The exact binding site has not bee characterized, at this time the most information we have is the binding region listed on the Datasheet of 195-223
Answered by: Tech Sales
Date published: 2021-06-25

I have purchased the following antibody (CTLA-4 Antibody (F-8): sc-376016) to assess the ctla-4 expression with western blot on un-activated whole human PBMC. I have used 80,000 cells but couldn’t get a signal. Can you please suggest if anyone

Asked by: IH87
Thank you for your question. Please contact our technical service team for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2021-02-18

Hope you could kindly let me know what is the dilution of CTLA-4 (F8) antibody to be used in a westernblot. Thanks

Asked by: Subhani
Thank you for your question. We recommend a starting dilution of 1:100 for this CTLA-4 antibody for Western Blot.
Answered by: Tech Service
Date published: 2019-09-23

Please send me the quote for CTLA-4 Antibody (F-8) sc-376016 200 µg/ml and how are you going to ship it.

Asked by: Neda Moatamed
Thank you for your question. Please contact our Customer Care department to request a quote. We will ship the antibody priority overnight for next day delivery. Please call us directly at (800) 457-3801 option 1, email customercare@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-22

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to HRP (sc-376016 HRP), phycoerythrin (sc-376016 PE), fluorescein (sc-376016 FITC), Alexa Fluor® 488 (sc-376016 AF488) or Alexa Fluor® 647 (sc-376016 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 1 out of 5 by from AF790 conjugated CLTA4When conjugated to AF790 this antibody does not yield specific staining for CTLA4 on mouse CD4+FOXP3+ cells from mouse splenocytes in our hands.
Date published: 2023-08-28
Rated 5 out of 5 by from IHC OF CTLA-4We used this antibody to do IHC for DLBCL, we can get a good result.
Date published: 2021-05-21
Rated 5 out of 5 by from Great AntibodyI bought this antibody a few weeks ago and it works great.
Date published: 2019-01-15
Rated 5 out of 5 by from Excellent result!I bought this product for a breast cancer proyect involving lymph nodes as well and I was very happy with the result obtained. Dilution 1/75 of clon F-8
Date published: 2018-12-08
Rated 4 out of 5 by from worked for westernsWorked for human overexpressed lysates in Hek293 cells
Date published: 2018-07-11
Rated 1 out of 5 by from CTLA-4 (F8) IF stainingUnfortunately, I used the CTLA-4 (F8) for mouse spleen and thymus IF staining, but it didn't work.
Date published: 2018-04-20
Rated 5 out of 5 by from the band is clearCTLA-4 antibody was diluted at 5% TBST with 1:500, I got an excellent result and this antibody was very stable.
Date published: 2017-11-01
Rated 3 out of 5 by from goodi used this antibody for western Blot at a concentration of 1:200, the bands were good but you may need to increase the concentration or protein loading
Date published: 2017-09-29
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CTLA-4 抗体 (F-8) is rated 4.2 out of 5 by 22.
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