CRSP70 Antibody (E-11): sc-137230

CRSP70 Antibody (E-11) is a mouse monoclonal IgG1 antibody that detects CRSP70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRSP70 plays a crucial role in transcription regulation by acting as a component of the CRSP (cofactor required for Sp1 activation) complex, which mediates signals between transcriptional activators and RNA polymerase. CRSP70 is primarily located in the nucleus, where CRSP70 participates in assembling high molecular weight coactivating complexes that facilitate gene transcription. CRSP70′s nuclear presence is vital for proper transcriptional machinery function, helping bridge transcription factors and the RNA polymerase II complex to enhance gene expression. CRSP70 shares structural similarities with elongation factors, underscoring CRSP70′s importance in transcription and highlighting CRSP70′s potential as a target for studying transcriptional regulation in various biological contexts. With broad species reactivity and versatility in multiple applications, anti-CRSP70 antibody (E-11) serves as an invaluable tool for researchers investigating gene regulation mechanisms.

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CRSP70 Antibody (E-11) References:

1. The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.  |  Marr, SK., et al. 2014. Mol Cell Biol. 34: 2710-20. PMID: 24820420
2. MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.  |  Takahashi, H., et al. 2015. Nat Commun. 6: 5941. PMID: 25575120
3. Cbx3 maintains lineage specificity during neural differentiation.  |  Huang, C., et al. 2017. Genes Dev. 31: 241-246. PMID: 28270516
4. Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.  |  Lens, Z., et al. 2017. J Mol Biol. 429: 3043-3055. PMID: 28893534
5. A simple protocol to purify human TFIID free of the MED26 subunit of mediator complex.  |  Xu, M. and Martinez, E. 2021. Protein Expr Purif. 184: 105887. PMID: 33836240
6. Structures of +1 nucleosome-bound PIC-Mediator complex.  |  Chen, X., et al. 2022. Science. 378: 62-68. PMID: 36201575
7. MED26-containing Mediator may orchestrate multiple transcription processes through organization of nuclear bodies.  |  Suzuki, H., et al. 2023. Bioessays. 45: e2200178. PMID: 36852638
8. An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II.  |  Zhao, H., et al. 2024. Mol Cell. 84: 2648-2664.e10. PMID: 38955181
9. 3D chromatin architecture, BRD4, and Mediator have distinct roles in regulating genome-wide transcriptional bursting and gene network.  |  Trzaskoma, P., et al. 2024. Sci Adv. 10: eadl4893. PMID: 39121214
10. Structural basis of the human transcriptional Mediator regulated by its dissociable kinase module.  |  Chao, TC., et al. 2024. Mol Cell. 84: 3932-3949.e10. PMID: 39321804

The preferred CRSP70 antibody is CRSP70 Antibody (G-11): sc-166614.