CRSP70 Antibody (G-11): sc-166614

CRSP70 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRSP70 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRSP70 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRSP70 plays a crucial role in transcription regulation within mammalian cells, functioning as part of the CRSP (cofactor required for Sp1 activation) complex, which mediates signals between transcriptional activators and RNA polymerase. This complex is vital for transcriptional activation by Sp1, and proper functioning is critical for gene expression regulation. CRSP70 is part of a multimeric complex consisting of nine distinct subunits, and CRSP70′s structure allows for integration of various signaling pathways that influence transcription. CRSP70′s ability to interact with other components of transcription machinery, including TAFIIs and larger coactivator complexes like ARC/DRI, underscores CRSP70′s importance in facilitating transcriptional responses to hormonal and metabolic signals. Given ubiquitous expression across different tissues, CRSP70 is integral to maintaining cellular homeostasis and responding to physiological changes, making CRSP70 a significant target for research in gene regulation and related fields.

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CRSP70 Antibody (G-11) References:

1. Composite co-activator ARC mediates chromatin-directed transcriptional activation.  |  Näär, AM., et al. 1999. Nature. 398: 828-32. PMID: 10235267
2. Purification of transcription cofactor complex CRSP.  |  Ryu, S. and Tjian, R. 1999. Proc Natl Acad Sci U S A. 96: 7137-42. PMID: 10377381
3. Three-dimensional structure of the human TFIID-IIA-IIB complex.  |  Andel, F., et al. 1999. Science. 286: 2153-6. PMID: 10591646
4. MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.  |  Ding, N., et al. 2009. J Biol Chem. 284: 2648-2656. PMID: 19049968
5. The structure of an Iws1/Spt6 complex reveals an interaction domain conserved in TFIIS, Elongin A and Med26.  |  Diebold, ML., et al. 2010. EMBO J. 29: 3979-91. PMID: 21057455
6. Human mediator subunit MED26 functions as a docking site for transcription elongation factors.  |  Takahashi, H., et al. 2011. Cell. 146: 92-104. PMID: 21729782
7. Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.  |  Vijayalingam, S. and Chinnadurai, G. 2013. J Virol. 87: 3425-34. PMID: 23302885
8. 1H, 15N and 13C assignments of the N-terminal domain of the Mediator complex subunit MED26.  |  Peruzzini, R., et al. 2016. Biomol NMR Assign. 10: 233-6. PMID: 26861138
9. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.  |  Kim, YJ., et al. 1994. Cell. 77: 599-608. PMID: 8187178
10. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.  |  Myers, LC., et al. 1998. Genes Dev. 12: 45-54. PMID: 9420330
11. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
12. The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1.  |  Ryu, S., et al. 1999. Nature. 397: 446-50. PMID: 9989412

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRSP70 Antibody (G-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CRSP70 Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRSP70 Antibody (G-11) Specifications

Alternate Product Name: CRSP70

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 373-600

Epitope Target: CRSP70

Epitope Species: human

Product Reactivity/Detection: CRSP70

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CRSP70 Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent