CRIK Antibody (E-6): sc-390437

CRIK Antibody (E-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects CRIK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRIK (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRIK, also known as Citron Rho-interacting kinase, plays a crucial role in the regulation of cytokinesis and is essential for proper development of the central nervous system. Located in the cytoplasm, CRIK interacts with Rho, a Ras-related small GTPase, to regulate actin-based cytoskeletal structures such as stress fibers and focal adhesions, which are vital for maintaining cell shape and facilitating cell division. CRIK consists of 2,027 amino acids and belongs to the protein kinase superfamily, specifically the AGC Ser/Thr protein kinase family, featuring an AGC-kinase C-terminal domain, a CNH domain, a PH domain, a phorbol-ester/DAG-type zinc finger, and a protein kinase domain. CRIK is required for the localization of KIF14 to the central spindle and midbody during cell division, and exists as four alternatively spliced isoforms encoded by a gene located on chromosome 12q24.23.

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CRIK Antibody (E-6) References:

1. Evidence of association between bipolar disorder and Citron on chromosome 12q24.  |  Lyons-Warren, A., et al. 2005. Mol Psychiatry. 10: 807-9. PMID: 15983625
2. Negative control of keratinocyte differentiation by Rho/CRIK signaling coupled with up-regulation of KyoT1/2 (FHL1) expression.  |  Grossi, M., et al. 2005. Proc Natl Acad Sci U S A. 102: 11313-8. PMID: 16061799
3. Dissecting the role of Rho-mediated signaling in contractile ring formation.  |  Kamijo, K., et al. 2006. Mol Biol Cell. 17: 43-55. PMID: 16236794
4. KIF14 and citron kinase act together to promote efficient cytokinesis.  |  Gruneberg, U., et al. 2006. J Cell Biol. 172: 363-72. PMID: 16431929
5. The Down syndrome critical region protein TTC3 inhibits neuronal differentiation via RhoA and Citron kinase.  |  Berto, G., et al. 2007. J Cell Sci. 120: 1859-67. PMID: 17488780
6. Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase.  |  Tan, I., et al. 2011. FEBS Lett. 585: 1260-8. PMID: 21457715
7. Citron Rho-interacting kinase mediates arsenite-induced decrease in endothelial nitric oxide synthase activity by increasing phosphorylation at threonine 497: Mechanism underlying arsenite-induced vascular dysfunction.  |  Seo, J., et al. 2016. Free Radic Biol Med. 90: 133-44. PMID: 26593676
8. Transcriptomic analysis of gene expression profiles of stomach carcinoma reveal abnormal expression of mitotic components.  |  Tong, H., et al. 2017. Life Sci. 170: 41-49. PMID: 27923621
9. Mutations of KIF14 cause primary microcephaly by impairing cytokinesis.  |  Moawia, A., et al. 2017. Ann Neurol. 82: 562-577. PMID: 28892560
10. Citron Rho-interacting kinase silencing causes cytokinesis failure and reduces tumor growth in multiple myeloma.  |  Sahin, I., et al. 2019. Blood Adv. 3: 995-1002. PMID: 30940634
11. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton.  |  Leung, T., et al. 1996. Mol Cell Biol. 16: 5313-27. PMID: 8816443
12. Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron.  |  Di Cunto, F., et al. 1998. J Biol Chem. 273: 29706-11. PMID: 9792683

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRIK Antibody (E-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-CRIK Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRIK Antibody (E-6) Specifications

Alternate Product Name: CRIK

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2036-2055

Epitope Target: CRIK

Epitope Species: mouse

Product Reactivity/Detection: CRIK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for CRIK Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin