CPSF2 Antibody (A-6) recommended for detection of CPSF2 of mouse, rat and human origin by WB, IP, IF and ELISA

CPSF2 Antibody (A-6): sc-398539

CPSF2 Antibody (A-6) is rated 5.0 out of 5 by 1.
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Datasheets

    • CPSF2 Antibody (A-6) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 194-493 mapping within an internal region of CPSF2 of human origin
    • recommended for detection of CPSF2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using CPSF2 (A-6): sc-398539?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great Western blot data of CPSF2 expression Great Western blot data of CPSF2 expression in HeLa, Ramos, HL-60 and BJAB nuclear extracts. -SCBT QC
Date published: 2013-05-08
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