CPSF2 Antibody (A-11): sc-165983
- CPSF2 Antibody (A-11) is a mouse monoclonal IgG1 κ CPSF2 antibody, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 258-295 within an internal region of CPSF2 of human origin
- CPSF2 Antibody (A-11) is recommended for detection of CPSF2 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- Anti-CPSF2 Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CPSF2 Antibody (A-11) for WB applications. These reagents are now offered in bundles with CPSF2 Antibody (A-11) (see ordering information below).
CPSF2 (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPSF2 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). CPSF2 (A-11) antibody is available as non-conjugated form, as well as multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. 3′ ends of eukaryotic mRNAs can undergo processing events that include endonucleolytic cleavage and polyadenylation. Cleavage and polyadenylation specificity factor (CPSF) mediates 3′ cleavage of the transcript and subsequent polyadenylation. CPSF contains four subunits and localizes to the nucleoplasm where CPSF recognizes the AAUAAA signal in pre-mRNA and interacts with other proteins to facilitate RNA cleavage and poly(A) synthesis (1,4,5). The human CPSF2 gene maps to chromosome 14q32.12 and encodes the second largest subunit of cleavage and polyadenylation specificity factor. U1 snRNP-A protein (U1A) interacts with and affects the activity of CPSF by stabilizing the interaction of CPSF with the AAUAAA-containing RNAs to increase the efficiency of polyadenylation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CPSF2 Antibody (A-11) References:
- A history of poly A sequences: from formation to factors to function. | Edmonds, M. 2002. Prog Nucleic Acid Res Mol Biol. 71: 285-389. PMID: 12102557
- The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. | Murthy, KG. and Manley, JL. 1995. Genes Dev. 9: 2672-83. PMID: 7590244
- Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro. | Lutz, CS., et al. 1996. Genes Dev. 10: 325-37. PMID: 8595883
- Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I. | Jenny, A., et al. 1996. Science. 274: 1514-7. PMID: 8929409
- The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. | Barabino, SM., et al. 1997. Genes Dev. 11: 1703-16. PMID: 9224719
- Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF. | Salinas, CA., et al. 1998. Mol Gen Genet. 257: 672-80. PMID: 9604891
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF2 Antibody (A-11) | sc-165983 | 200 µg/ml | $322.00 | |||
CPSF2 Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-528887 | 200 µg Ab; 10 µg BP | $361.00 | |||
CPSF2 Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-521475 | 200 µg Ab, 40 µg BP | $361.00 | |||
CPSF2 Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-543193 | 200 µg Ab; 20 µg BP | $361.00 | |||
CPSF2 Antibody (A-11) AC | sc-165983 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CPSF2 Antibody (A-11) HRP | sc-165983 HRP | 200 µg/ml | $322.00 | |||
CPSF2 Antibody (A-11) FITC | sc-165983 FITC | 200 µg/ml | $336.00 | |||
CPSF2 Antibody (A-11) PE | sc-165983 PE | 200 µg/ml | $349.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 488 | sc-165983 AF488 | 200 µg/ml | $364.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 546 | sc-165983 AF546 | 200 µg/ml | $364.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 594 | sc-165983 AF594 | 200 µg/ml | $364.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 647 | sc-165983 AF647 | 200 µg/ml | $364.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 680 | sc-165983 AF680 | 200 µg/ml | $364.00 | |||
CPSF2 Antibody (A-11) Alexa Fluor® 790 | sc-165983 AF790 | 200 µg/ml | $364.00 | |||
CPSF2 (A-11) Neutralizing Peptide | sc-165983 P | 100 µg/0.5 ml | $69.00 |