cPLA₂ Antibody (4-4B-3C): sc-454

cPLA2 Antibody (4-4B-3C) is a mouse monoclonal IgG2b kappa light chain antibody that detects cytosolic phospholipase A2 (cPLA2) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). cPLA2 monoclonal antibody (4-4B-3C) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. cPLA2 plays a crucial role in lipid metabolism by hydrolyzing the sn-2-acyl ester bond in glycerophospholipids, which leads to the release of free fatty acids, including arachidonic acid. This process is vital as arachidonic acid serves as a precursor for the synthesis of eicosanoids, such as prostaglandins and leukotrienes, which are key mediators in inflammatory responses. The ability of cPLA2 to preferentially release arachidonic acid from membrane phospholipids highlights its importance in regulating inflammatory processes and cellular signaling pathways. cPLA2 (4-4B-3C) antibody enables researchers to effectively study the dynamics of cPLA2 in various biological contexts, enhancing our understanding of its role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cPLA₂ Antibody (4-4B-3C) References:

1. Regulation of extracellular phospholipase A2 activity: implications for inflammatory diseases.  |  Mukherjee, AB., et al. 1992. DNA Cell Biol. 11: 233-43. PMID: 1567556
2. Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2.  |  Sharp, JD., et al. 1991. J Biol Chem. 266: 14850-3. PMID: 1869522
3. A novel bifunctional mechanism of surface recognition by phospholipase A2.  |  Heinrikson, RL. and Kézdy, FJ. 1990. Adv Exp Med Biol. 279: 37-47. PMID: 2096699
4. Purification of a phospholipase A2 from human monocytic leukemic U937 cells. Calcium-dependent activation and membrane association.  |  Diez, E. and Mong, S. 1990. J Biol Chem. 265: 14654-61. PMID: 2201689
5. Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937.  |  Clark, JD., et al. 1990. Proc Natl Acad Sci U S A. 87: 7708-12. PMID: 2217203
6. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma.  |  Sarkar, C., et al. 2020. Autophagy. 16: 466-485. PMID: 31238788
7. Properties and purification of an arachidonoyl-hydrolyzing phospholipase A2 from a macrophage cell line, RAW 264.7.  |  Leslie, CC., et al. 1988. Biochim Biophys Acta. 963: 476-92. PMID: 3143418
8. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS.  |  Chao, CC., et al. 2019. Cell. 179: 1483-1498.e22. PMID: 31813625
9. Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.  |  Lou, J., et al. 2022. Autophagy. 18: 1841-1863. PMID: 34872436
10. HDAC5 Loss Enhances Phospholipid-Derived Arachidonic Acid Generation and Confers Sensitivity to cPLA2 Inhibition in Pancreatic Cancer.  |  Pan, P., et al. 2022. Cancer Res. 82: 4542-4554. PMID: 36102738
11. Amino acid sequence of phospholipase A2-alpha from the venom of Crotalus adamanteus. A new classification of phospholipases A2 based upon structural determinants.  |  Heinrikson, RL., et al. 1977. J Biol Chem. 252: 4913-21. PMID: 873920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cPLA₂ Antibody (4-4B-3C) has 135 citations in a variety of scientific publications.

Primary antibodies like Anti-cPLA₂ Antibody (4-4B-3C) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cPLA₂ Antibody (4-4B-3C) Specifications

Alternate Product Name: phospholipase A2 group IVA (PLA2G4A)

Product Clone Name: 4-4B-3C

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-216

Epitope Target: cPLA2

Epitope Species: human

Product Reactivity/Detection: cytosolic PLA₂

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 135 citations in various scientific publications

Available Conjugates/Forms for cPLA₂ Antibody (4-4B-3C): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin