CPI-17 Antibody (F-4): sc-48406

CPI-17 Antibody (F-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects CPI-17 protein of mouse, rat, human, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPI-17 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPI-17 plays a crucial role as a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase, which is essential for regulating smooth muscle contraction. Originally identified as a protein kinase C-potentiated inhibitor of protein phosphatase-1, CPI-17 is predominantly expressed in smooth muscle tissues. Phosphorylation at Threonine 38, mediated by protein kinase C or Rho-kinase, significantly enhances CPI-17′s inhibitory potency towards myosin phosphatase, thereby contributing to calcium sensitization during smooth muscle contraction. Additionally, CPI-17 is phosphorylated at Serine 12 and Threonine 38 by the MYPT-associated kinase, M110 kinase, highlighting complex regulatory mechanisms. This multifaceted role in smooth muscle physiology underscores CPI-17′s importance in various physiological and pathological processes, making anti-CPI-17 antibody (F-4) an invaluable tool for researchers studying smooth muscle function and regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPI-17 Antibody (F-4) References:

1. Phosphorylation of CPI-17, an inhibitory phosphoprotein of smooth muscle myosin phosphatase, by Rho-kinase.  |  Koyama, M., et al. 2000. FEBS Lett. 475: 197-200. PMID: 10869555
2. Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase.  |  Yamawaki, K., et al. 2001. Biochem Biophys Res Commun. 285: 1040-5. PMID: 11467857
3. Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.  |  Deng, JT., et al. 2002. Biochem J. 367: 517-24. PMID: 12144526
4. Role of CPI-17 in the regulation of endothelial cytoskeleton.  |  Kolosova, IA., et al. 2004. Am J Physiol Lung Cell Mol Physiol. 287: L970-80. PMID: 15234908
5. G protein-mediated Ca²+-sensitization of CPI-17 phosphorylation in arterial smooth muscle.  |  Kitazawa, T. 2010. Biochem Biophys Res Commun. 401: 75-8. PMID: 20833141
6. Synchronous phosphorylation of CPI-17 and MYPT1 is essential for inducing Ca(2+) sensitization in intestinal smooth muscle.  |  Mori, D., et al. 2011. Neurogastroenterol Motil. 23: 1111-22. PMID: 22004286
7. Smooth muscle-selective CPI-17 expression increases vascular smooth muscle contraction and blood pressure.  |  Su, W., et al. 2013. Am J Physiol Heart Circ Physiol. 305: H104-13. PMID: 23604714
8. Relationship between uterine smooth muscular CPI-17-signal pathway-mediated Ca2+ sensitivity changes and uterine atony-induced postpartum haemorrhage.  |  Liao, Q., et al. 2019. J Obstet Gynaecol. 39: 302-307. PMID: 30428734
9. Transcriptional Suppression of CPI-17 Gene Expression in Vascular Smooth Muscle Cells by Tumor Necrosis Factor, Krüppel-Like Factor 4, and Sp1 Is Associated with Lipopolysaccharide-Induced Vascular Hypocontractility, Hypotension, and Mortality.  |  Zhao, G., et al. 2019. Mol Cell Biol. 39: PMID: 30936247
10. Possible roles of N- and C-terminal unstructured tails of CPI-17 in regulating Ca2+ sensitization force of smooth muscle.  |  Eto, M., et al. 2022. J Smooth Muscle Res. 58: 22-33. PMID: 35418530

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPI-17 Antibody (F-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CPI-17 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPI-17 Antibody (F-4) Specifications

Alternate Product Name: PPP1R14A

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 88-147

Epitope Target: CPI-17

Epitope Species: human

Product Reactivity/Detection: CPI-17

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CPI-17 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CPI-17 antibody is CPI-17 Antibody (C-1): sc-365841.