COL7A1 Antibody (LH7.2) recommended for detection of Collagen Type VII of mouse, rat and human origin by WB, IF and IHC(P)

COL7A1 Antibody (LH7.2): sc-53226

COL7A1 Antibody (LH7.2) is rated 4.7 out of 5 by 3.
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Datasheets

    • COL7A1 Antibody (LH7.2) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • raised against neonatal skin of human origin
    • recommended for detection of Collagen Type VII of mouse, rat and human origin by WB, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See COL7A1 (4D2): sc-33710 for COL7A1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL7A1 (LH7.2): sc-53226.
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Do you have a protocol for western blot assays in human cells

Asked by: Anonymous
Thank you for your question. Yes, we do have a protocol for Western blot. You can find it here: https://www.scbt.com/resources/protocols/western-immuno-blotting For this antibody we recommend a starting dilution of 1:100.
Answered by: Tech Service
Date published: 2019-12-20

I am using COL7A1 (LH7.2): sc-53226 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect antibody Works perfectly on frozen tissue section. Very specific, with stongs signals
Date published: 2018-06-07
Rated 5 out of 5 by from Worked great in IP staining images We used this antibody for Imumunoperoxidase staining and were able to take nice pictures of stained cells
Date published: 2017-01-27
Rated 4 out of 5 by from Nice Nice, specific, staining in paraffin-embedded human kidney samples. -SCBT QC
Date published: 2015-02-01
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