Anti-COL7A1 Antibody (4D2) is a mouse monoclonal IgG1 (kappa light chain) COL7A1 antibody provided at 200 µg/ml
raised against SDS-insoluble proteins of normal human skin
COL7A1 Antibody (4D2) is recommended for detection of Collagen Type VII of human origin by WB, IP, IF and IHC(P)
Anti-COL7A1 Antibody (4D2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL7A1 (4D2): sc-33710.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
COL7A1 Antibody (4D2) is a high quality monoclonal COL7A1 antibody (also designated COL7A1 antibody) suitable for the detection of the COL7A1 protein of human origin. COL7A1 Antibody (4D2) is available as both the non-conjugated anti-COL7A1 antibody form, as well as multiple conjugated forms of anti-COL7A1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Do you know if this antibody (COL7A1 Antibody (4D2): sc-33710) reacts with mouse, or only human? We are searching for an anti-COL7A1 antibody that will NOT recognized mouse collagen VII, only human, and is conjugated. Thanks for the help.
Asked by: Dorothy
Thank you for your question. We recommend sc-33710:COL7A1 (4D2) for use with human samples. The exact binding site of sc-33710:COL7A1 (4D2) has not been characterized and, therefore, unable to determine homolgy with mouse COL7A1. We offer sc-33710:COL7A1 (4D2) conjugated to HRP and a variety of fluorochromes. If you are interested, we are providing complimentary samples of the unconjugated version of this antibody for a limited time. Please contact technical service for further information.
Answered by: Santa Cruz TS
Date published: 2017-05-19
Can COL7A1 (4D2): sc-33710 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, COL7A1 (4D2): sc-33710 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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