COL7A1 Antibody (4D2) recommended for detection of Collagen Type VII of human origin by WB, IP, IF and IHC(P)

COL7A1 Antibody (4D2): sc-33710

COL7A1 Antibody (4D2) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-COL7A1 Antibody (4D2) is a mouse monoclonal IgG1 (kappa light chain) COL7A1 antibody provided at 200 µg/ml
    • raised against SDS-insoluble proteins of normal human skin
    • COL7A1 Antibody (4D2) is recommended for detection of Collagen Type VII of human origin by WB, IP, IF and IHC(P)
    • Anti-COL7A1 Antibody (4D2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL7A1 (4D2): sc-33710.
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Do you know if this antibody (COL7A1 Antibody (4D2): sc-33710) reacts with mouse, or only human? We are searching for an anti-COL7A1 antibody that will NOT recognized mouse collagen VII, only human, and is conjugated. Thanks for the help.

Asked by: Dorothy
Thank you for your question. We recommend sc-33710:COL7A1 (4D2) for use with human samples. The exact binding site of sc-33710:COL7A1 (4D2) has not been characterized and, therefore, unable to determine homolgy with mouse COL7A1. We offer sc-33710:COL7A1 (4D2) conjugated to HRP and a variety of fluorochromes. If you are interested, we are providing complimentary samples of the unconjugated version of this antibody for a limited time. Please contact technical service for further information.
Answered by: Santa Cruz TS
Date published: 2017-05-19

Can COL7A1 (4D2): sc-33710 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, COL7A1 (4D2): sc-33710 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong IF staining We used this antibody for IF staining and were able to produce very sharp images showing membrane localization
Date published: 2017-01-25
Rated 5 out of 5 by from IHC on paraffin embedded sections of human IHC on paraffin embedded sections of human skin (dilution 1/50) - Published data
Date published: 2015-08-07
Rated 5 out of 5 by from Antibody works great for IHC paraffin Antibody works great for IHC paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-20
Rated 5 out of 5 by from Worked well for immunofluorescence staining Worked well for immunofluorescence staining of normal human cornea frozen section. -SCBT QC
Date published: 2015-01-11
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