COL6A3 Antibody (A-5)recommended for detection of Collagen α3 Type VI isoforms 1 and 2 of human origin by WB, IP, IF and ELISA

COL6A3 Antibody (A-5): sc-515335

COL6A3 Antibody (A-5) is rated 2.5 out of 5 by 2.
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Datasheets

    • COL6A3 Antibody (A-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 301-600 mapping within an internal region of Collagen α3 Type VI of human origin
    • COL6A3 Antibody (A-5) is recommended for detection of Collagen α3 Type VI isoforms 1 and 2 of human origin by WB, IP, IF and ELISA
    • COL6A3 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL6A3 (A-5): sc-515335.
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What is the recommended starting dilution for Western blot for sc-515335: COL6A3 (A-5) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 4 out of 5 by from works good in IHC i tried this sample antibody in human FFPE skin sections and it worked well. It showed a very strong signal as well as some backgrounds. Overall, it works good.
Date published: 2017-07-05
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