CNPase Antibody (B-9): sc-166254

CNPase Antibody (B-9) is a mouse monoclonal IgG2b antibody that detects 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CNPase plays a crucial role as a membrane-bound enzyme that links tubulin to membranes, thereby regulating the distribution of cytoplasmic microtubules, which is essential for maintaining cellular structure and function. CNPase acts as a microtubule-associated protein, promoting microtubule assembly, particularly through its C-terminal region. CNPase is predominantly found in the central nervous system, where CNPase is highly concentrated in myelin, as well as in the outer segments of photoreceptors in the retina, highlighting CNPase′s importance in neuronal health and visual function. Notably, disturbances in CNPase activity, such as those caused by acute lead intoxication, can lead to significant alterations in myelin morphology, underscoring CNPase′s critical role in neuroprotection and myelination. Anti-CNPase antibody (B-9) is an invaluable tool for researchers investigating the dynamics of microtubule organization and the implications of CNPase in various neurological conditions.

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CNPase Antibody (B-9) References:

1. T cell response to 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in multiple sclerosis patients.  |  Muraro, PA., et al. 2002. J Neuroimmunol. 130: 233-42. PMID: 12225906
2. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in the developing olfactory bulb and subventricular zone rostral extension.  |  Gomes, SS., et al. 2003. J Neurosci Res. 73: 471-80. PMID: 12898532
3. Synaptin, Na+,K+ ATPase, LDH, CNPase and cytochrome-c oxidase in adult rat brain synaptic vesicle fractions.  |  Reeber, A., et al. 1978. FEBS Lett. 86: 171-3. PMID: 203483
4. Hexachlorophene and cuprizone induce the spongy change of the developing rat brain by different mechanisms: the role of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase).  |  Kanno, T., et al. 2012. J Vet Med Sci. 74: 837-43. PMID: 22313968
5. EAE serum decreases CNPase activity in cultures of B104 cells.  |  Elkin, R., et al. 1988. Ann N Y Acad Sci. 540: 396-7. PMID: 2462816
6. CNPase activity in the vertebrate retina, retinal pigmented epithelium, and choroid.  |  Heath, AR. and Hindman, HM. 1986. J Exp Zool. 238: 183-91. PMID: 3011952
7. Sirt3 increases CNPase enzymatic activity through deacetylation and facilitating substrate accessibility.  |  Wang, D., et al. 2021. Biochem Biophys Res Commun. 571: 181-187. PMID: 34330062
8. CNPase, a 2',3'-Cyclic-nucleotide 3'-phosphodiesterase, as a Therapeutic Target to Attenuate Cardiac Hypertrophy by Enhancing Mitochondrial Energy Production.  |  Tan, KS., et al. 2021. Int J Mol Sci. 22: PMID: 34639145
9. Alteration of 5'-nucleotidase and Na+,K+-ATPase in central and peripheral nervous tissue from dysmyelinating mutants (jimpy, quaking, trembler, shiverer, and mld). Comparison with CNPase in the developing sciatic nerve from trembler.  |  Bourre, JM., et al. 1982. J Neurochem. 38: 643-9. PMID: 6276507
10. Dissociation of CNPase dimer into catalytically active monomers by 1,4-dithiothreitol and urea. Protein-promoted reassembly.  |  Müller, HW. 1982. FEBS Lett. 144: 77-80. PMID: 6286358

The preferred CNPase antibody is CNPase Antibody (H-2): sc-166558.