CNPase Antibody (H-2): sc-166558

CNPase Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CNPase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CNPase monoclonal antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 2′,3′-cyclic nucleotide-3′-phosphodiesterase (CNPase) protein plays a crucial role in the central nervous system as a membrane-bound enzyme that links tubulin to membranes, thereby regulating the distribution of cytoplasmic microtubules. This function is vital for maintaining the structural integrity and proper functioning of neurons, as microtubules are essential for intracellular transport and the overall architecture of the cell. CNPase acts as a microtubule-associated protein, promoting microtubule assembly, with this activity localized to the C-terminus of the enzyme. CNPase is predominantly found in high concentrations within central nervous system myelin and the outer segments of photoreceptors in the retina, highlighting its importance in both myelination and visual processes. Disturbances in CNPase activity, such as those caused by acute lead intoxication, can lead to significant alterations in myelin morphology, underscoring CNPase′s critical role in neural health and function.

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CNPase Antibody (H-2) References:

1. Acute lead intoxication in vivo affects myelin membrane morphology and CNPase activity.  |  Dabrowska-Bouta, B., et al. 2000. Exp Toxicol Pathol. 52: 257-63. PMID: 10930127
2. 2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin.  |  Bifulco, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 1807-12. PMID: 11842207
3. CNPase expression in olfactory ensheathing cells.  |  Radtke, C., et al. 2011. J Biomed Biotechnol. 2011: 608496. PMID: 22174557
4. Role of CNPase in the oligodendrocytic extracellular 2',3'-cAMP-adenosine pathway.  |  Verrier, JD., et al. 2013. Glia. 61: 1595-606. PMID: 23922219
5. Enriched environment induces higher CNPase positive cells in aged rat hippocampus.  |  Zhao, YY., et al. 2013. Neurosci Lett. 555: 177-81. PMID: 24076141
6. Monoclonal antibody production to human and bovine 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase): high-specificity recognition in whole brain acetone powders and conservation of sequence between CNP1 and CNP2.  |  Sprinkle, TJ., et al. 1987. Brain Res. 426: 349-57. PMID: 2446713
7. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and its roles in activated microglia in vivo and in vitro.  |  Yang, L., et al. 2014. J Neuroinflammation. 11: 148. PMID: 25148928
8. Administration of vitamin D3 induces CNPase and myelin oligodendrocyte glycoprotein expression in the cerebral cortex of the murine model of cuprizone-induced demyelination.  |  Mashayekhi, F. and Salehi, Z. 2016. Folia Neuropathol. 54: 259-264. PMID: 27764518
9. Molecular structure, localization, and possible functions of the myelin-associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase.  |  Vogel, US. and Thompson, RJ. 1988. J Neurochem. 50: 1667-77. PMID: 2836557
10. Supplemental study on 2', 3'-Cyclic Nucleotide 3'-Phosphodiesterase (CNPase) activity in developing rat spinal cord lesions induced by hexachlorophene and cuprizone.  |  Kanno, T., et al. 2019. J Vet Med Sci. 81: 1368-1372. PMID: 31447458
11. EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone.  |  Lira-Diaz, E., et al. 2022. Life (Basel). 12: PMID: 35330085
12. Nanobodies against the myelin enzyme CNPase as tools for structural and functional studies.  |  Markusson, S., et al. 2025. J Neurochem. 169: e16274. PMID: 39655780

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CNPase Antibody (H-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CNPase Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CNPase Antibody (H-2) Specifications

Alternate Product Name: CNPase

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 363-380

Epitope Target: CNPase

Epitope Species: human

Product Reactivity/Detection: CNPase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CNPase Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin