CMBL Antibody (B-5): sc-514233
- CMBL Antibody (B-5) is a mouse monoclonal IgG2b κ CMBL antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 77-98 within an internal region of CMBL of human origin
- CMBL Antibody (B-5) is recommended for detection of CMBL of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CMBL Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CMBL Antibody (B-5) for WB applications. These reagents are now offered in bundles with CMBL Antibody (B-5) (see ordering information below).
QUICK LINKS
SEE ALSO...
CMBL Antibody (B-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects CMBL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CMBL (B-5) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CMBL protein, or Carboxymethylenebutenolidase homolog, is a 245 amino acid cytosolic protein and cysteine hydrolase that plays a crucial role in cleaving cyclic esters, which is important for various metabolic processes. As a member of the dienelactone hydrolase family, CMBL serves as the human counterpart to the Pseudomonas dienelactone hydrolase, contributing to the bacterial halocatechol degradation pathway. CMBL is highly expressed in the liver, colon, kidney, and small intestine, where CMBL functions as a bioactivating enzyme for the prodrug angiotensin II type 1 receptor antagonist, olmesartan medoxomil. This enzymatic activity enhances the therapeutic efficacy of olmesartan, which is used in treating hypertension. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene located on human chromosome 5p15.2, a region critical for various genetic functions and disorders. Deletion of the p arm of chromosome 5 is associated with Cri du chat syndrome, while abnormalities in the q arm are frequently observed in therapy-related acute myelogenous leukemias and myelodysplastic syndromes, highlighting CMBL′s importance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CMBL Antibody (B-5) References:
- Antagonizing the angiotensin II subtype 1 receptor: a focus on olmesartan medoxomil. | Nussberger, J. and Koike, H. 2004. Clin Ther. 26 Suppl A: A12-20. PMID: 15291375
- A review of the structural and functional features of olmesartan medoxomil, an angiotensin receptor blocker. | Mire, DE., et al. 2005. J Cardiovasc Pharmacol. 46: 585-93. PMID: 16220064
- Haematological & molecular profile of acute myelogenous leukaemia in India. | Sazawal, S., et al. 2009. Indian J Med Res. 129: 256-61. PMID: 19491417
- Superior outcome with hypomethylating therapy in patients with acute myeloid leukemia and high-risk myelodysplastic syndrome and chromosome 5 and 7 abnormalities. | Ravandi, F., et al. 2009. Cancer. 115: 5746-51. PMID: 19795507
- Characterization of a de novo complex chromosomal rearrangement in a patient with cri-du-chat and trisomy 5p syndromes. | Vera-Carbonell, A., et al. 2009. Am J Med Genet A. 149A: 2513-21. PMID: 19842199
- Human carboxymethylenebutenolidase as a bioactivating hydrolase of olmesartan medoxomil in liver and intestine. | Ishizuka, T., et al. 2010. J Biol Chem. 285: 11892-902. PMID: 20177059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CMBL Antibody (B-5) | sc-514233 | 200 µg/ml | $322.00 | |||
CMBL Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-524656 | 200 µg Ab, 40 µg BP | $361.00 | |||
CMBL Antibody (B-5): m-IgG2b BP-HRP Bundle | sc-549171 | 200 µg Ab; 10 µg BP | $361.00 | |||
CMBL Antibody (B-5) AC | sc-514233 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CMBL Antibody (B-5) HRP | sc-514233 HRP | 200 µg/ml | $322.00 | |||
CMBL Antibody (B-5) FITC | sc-514233 FITC | 200 µg/ml | $336.00 | |||
CMBL Antibody (B-5) PE | sc-514233 PE | 200 µg/ml | $349.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 488 | sc-514233 AF488 | 200 µg/ml | $364.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 546 | sc-514233 AF546 | 200 µg/ml | $364.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 594 | sc-514233 AF594 | 200 µg/ml | $364.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 647 | sc-514233 AF647 | 200 µg/ml | $364.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 680 | sc-514233 AF680 | 200 µg/ml | $364.00 | |||
CMBL Antibody (B-5) Alexa Fluor® 790 | sc-514233 AF790 | 200 µg/ml | $364.00 | |||
CMBL (B-5) Neutralizing Peptide | sc-514233 P | 100 µg/0.5 ml | $69.00 |