CHRNB4 Antibody (E-3): sc-514315
- CHRNB4 Antibody (E-3) is a mouse monoclonal IgG1 κ CHRNB4 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 336-432 mapping near the C-terminus within a cytoplasmic domain of CHRNB4 of human origin
- CHRNB4 Antibody (E-3) is recommended for detection of CHRNB4 of human origin by WB, IP, IF and ELISA
- Anti-CHRNB4 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CHRNB4 Antibody (E-3) for WB applications. These reagents are now offered in bundles with CHRNB4 Antibody (E-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
CHRNB4 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CHRNB4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CHRNB4 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CHRNB4 (E-3) antibody recognizes CHRNB4, a 498-amino acid protein that serves as a crucial component of the ligand-gated ion channel family, specifically functioning as a subunit of nicotinic acetylcholine receptors. These receptors are vital for synaptic transmission, mediating fast response to neurotransmitters like acetylcholine at the neuromuscular junction and in the central nervous system. CHRNB4 contributes to forming the receptor′s transmembrane aqueous pore, allowing ions to flow in response to acetylcholine binding, which is essential for muscle contraction and neuronal signaling. CHRNB4 is also implicated in the glutamatergic pathway and may play a role in nicotine dependence, highlighting its importance in both neuromuscular function and potential addiction mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHRNB4 Antibody (E-3) References:
- Deletion of the alpha7, beta2, or beta4 nicotinic receptor subunit genes identifies highly expressed subtypes with relatively low affinity for [3H]epibatidine. | Marks, MJ., et al. 2006. Mol Pharmacol. 70: 947-59. PMID: 16728647
- Differential brain transcriptome of beta4 nAChR subunit-deficient mice: is it the effect of the null mutation or the background strain? | Kedmi, M. and Orr-Urtreger, A. 2007. Physiol Genomics. 28: 213-22. PMID: 16985005
- Further evidence of genetic heterogeneity in families with autosomal dominant nocturnal frontal lobe epilepsy. | De Marco, EV., et al. 2007. Epilepsy Res. 74: 70-3. PMID: 17324557
- No evidence for association between 19 cholinergic genes and bipolar disorder. | Shi, J., et al. 2007. Am J Med Genet B Neuropsychiatr Genet. 144B: 715-23. PMID: 17373692
- Cellular prion protein co-localizes with nAChR beta4 subunit in brain and gastrointestinal tract. | Petrakis, S., et al. 2008. Eur J Neurosci. 27: 612-20. PMID: 18279314
- A candidate gene approach identifies the CHRNA5-A3-B4 region as a risk factor for age-dependent nicotine addiction. | Weiss, RB., et al. 2008. PLoS Genet. 4: e1000125. PMID: 18618000
- A common genetic variant in the 15q24 nicotinic acetylcholine receptor gene cluster (CHRNA5-CHRNA3-CHRNB4) is associated with a reduced ability of women to quit smoking in pregnancy. | Freathy, RM., et al. 2009. Hum Mol Genet. 18: 2922-7. PMID: 19429911
- Rare missense variants of neuronal nicotinic acetylcholine receptor altering receptor function are associated with sporadic amyotrophic lateral sclerosis. | Sabatelli, M., et al. 2009. Hum Mol Genet. 18: 3997-4006. PMID: 19628475
- Association and interaction analysis of variants in CHRNA5/CHRNA3/CHRNB4 gene cluster with nicotine dependence in African and European Americans. | Li, MD., et al. 2010. Am J Med Genet B Neuropsychiatr Genet. 153B: 745-56. PMID: 19859904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHRNB4 Antibody (E-3) | sc-514315 | 200 µg/ml | $322.00 | |||
CHRNB4 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-531089 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHRNB4 Antibody (E-3): m-IgGκ BP-HRP Bundle | sc-524684 | 200 µg Ab, 40 µg BP | $361.00 | |||
CHRNB4 Antibody (E-3): m-IgG1 BP-HRP Bundle | sc-544391 | 200 µg Ab; 20 µg BP | $361.00 | |||
CHRNB4 Antibody (E-3) AC | sc-514315 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CHRNB4 Antibody (E-3) HRP | sc-514315 HRP | 200 µg/ml | $322.00 | |||
CHRNB4 Antibody (E-3) FITC | sc-514315 FITC | 200 µg/ml | $336.00 | |||
CHRNB4 Antibody (E-3) PE | sc-514315 PE | 200 µg/ml | $349.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 488 | sc-514315 AF488 | 200 µg/ml | $364.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 546 | sc-514315 AF546 | 200 µg/ml | $364.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 594 | sc-514315 AF594 | 200 µg/ml | $364.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 647 | sc-514315 AF647 | 200 µg/ml | $364.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 680 | sc-514315 AF680 | 200 µg/ml | $364.00 | |||
CHRNB4 Antibody (E-3) Alexa Fluor® 790 | sc-514315 AF790 | 200 µg/ml | $364.00 |