CH25H Antibody (1G8): sc-293256

CH25H Antibody (1G8) is a mouse monoclonal IgG2b kappa light chain antibody that detects CH25H protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CH25H antibody (1G8) is available as the non-conjugated format. CH25H, or Cholesterol 25-hydroxylase, is a crucial 272 amino acid endoplasmic membrane protein that plays a significant role in cholesterol and lipid metabolism. CH25H (1G8) monoclonal antibody recognizes a protein essential for catalyzing the conversion of cholesterol into 25-hydroxycholesterol, a process that not only regulates cholesterol biosynthetic enzymes but also influences the overall lipid metabolic pathway. CH25H protein′s importance lies in its ability to synthesize a corepressor that inhibits the processing of sterol regulatory element binding protein (SREBP), thereby maintaining lipid homeostasis. Additionally, CH25H utilizes diiron cofactors to facilitate the hydroxylation of hydrophobic substrates, underscoring its intricate role in cellular lipid regulation and the potential implications for metabolic disorders.

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CH25H Antibody (1G8) References:

1. Association analysis of genes involved in cholesterol metabolism located within the linkage region on chromosome 10 and Alzheimer's disease.  |  Riemenschneider, M., et al. 2004. Neurobiol Aging. 25: 1305-8. PMID: 15465627
2. Association studies of cholesterol metabolism genes (CH25H, ABCA1 and CH24H) in Alzheimer's disease.  |  Shibata, N., et al. 2006. Neurosci Lett. 391: 142-6. PMID: 16157450
3. Regulation of cholesterol 25-hydroxylase expression by vitamin D3 metabolites in human prostate stromal cells.  |  Wang, JH. and Tuohimaa, P. 2006. Biochem Biophys Res Commun. 345: 720-5. PMID: 16696936
4. Cholesterol 25-hydroxylase on chromosome 10q is a susceptibility gene for sporadic Alzheimer's disease.  |  Papassotiropoulos, A., et al. 2005. Neurodegener Dis. 2: 233-41. PMID: 16909003
5. Targeting the prostaglandin D2 receptors DP and CRTH2 for treatment of inflammation.  |  Ulven, T. and Kostenis, E. 2006. Curr Top Med Chem. 6: 1427-44. PMID: 16918458
6. Convergence of genes implicated in Alzheimer's disease on the cerebral cholesterol shuttle: APP, cholesterol, lipoproteins, and atherosclerosis.  |  Carter, CJ. 2007. Neurochem Int. 50: 12-38. PMID: 16973241
7. Association studies of 23 positional/functional candidate genes on chromosome 10 in late-onset Alzheimer's disease.  |  Morgan, AR., et al. 2007. Am J Med Genet B Neuropsychiatr Genet. 144B: 762-70. PMID: 17373700
8. Assessment of Alzheimer's disease case-control associations using family-based methods.  |  Schjeide, BM., et al. 2009. Neurogenetics. 10: 19-25. PMID: 18830724
9. Discovery of potent, selective, and orally bioavailable alkynylphenoxyacetic acid CRTH2 (DP2) receptor antagonists for the treatment of allergic inflammatory diseases.  |  Crosignani, S., et al. 2011. J Med Chem. 54: 7299-317. PMID: 21916510
10. KLRG1 and NKp46 discriminate subpopulations of human CD117+CRTH2- ILCs biased toward ILC2 or ILC3.  |  Nagasawa, M., et al. 2019. J Exp Med. 216: 1762-1776. PMID: 31201208
11. Molecular basis for lipid recognition by the prostaglandin D2 receptor CRTH2.  |  Liu, H., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34341104
12. Molecular basis of lipid and ligand regulation of prostaglandin receptor DP2.  |  Xu, J., et al. 2024. Proc Natl Acad Sci U S A. 121: e2403304121. PMID: 39665758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CH25H Antibody (1G8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CH25H Antibody (1G8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CH25H Antibody (1G8) Specifications

Alternate Product Name: CH25H

Product Clone Name: 1G8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 142-247

Epitope Target: CH25H

Epitope Species: human

Product Reactivity/Detection: CH25H

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CH25H Antibody (1G8): N/A