cGKII Antibody (D-3): sc-393126

cGKII Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects cGKII protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). cGKII (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cGKII, also known as cGMP-dependent protein kinase type II, functions as a receptor for intracellular cyclic guanosine monophosphate (cGMP), mediating various physiological responses, particularly in regulating ion transport systems in the intestine. cGKII expression occurs in key tissues such as the small intestine, colon, prostate, and human brain, with its gene located on chromosome 4q21.1. A conserved leucine zipper motif at the amino-terminus facilitates protein-protein interactions. Myristoylation at the penultimate glycine enables membrane localization, compensating for the absence of hydrophobic transmembrane domains. This cytosol-to-membrane translocation is essential for regulating intestinal ion transport, emphasizing the importance of structural and functional integrity in physiological processes.

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cGKII Antibody (D-3) References:

1. Cyclic nucleotide-dependent protein kinases: intracellular receptors for cAMP and cGMP action.  |  Francis, SH. and Corbin, JD. 1999. Crit Rev Clin Lab Sci. 36: 275-328. PMID: 10486703
2. Autophosphorylation of cGMP-dependent protein kinase type II.  |  Vaandrager, AB., et al. 2003. J Biol Chem. 278: 28651-8. PMID: 12764134
3. A GluR1-cGKII interaction regulates AMPA receptor trafficking.  |  Serulle, Y., et al. 2007. Neuron. 56: 670-88. PMID: 18031684
4. Fusion of PRKG2 and SPTBN1 to the platelet-derived growth factor receptor beta gene (PDGFRB) in imatinib-responsive atypical myeloproliferative disorders.  |  Gallagher, G., et al. 2008. Cancer Genet Cytogenet. 181: 46-51. PMID: 18262053
5. Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site.  |  Chen, T., et al. 2015. J Biol Chem. 290: 1952-65. PMID: 25480791
6. Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLCβ1-Ca2+-Mediated Endoplasmic Reticulum Stress.  |  Jia, T., et al. 2021. Oxid Med Cell Longev. 2021: 5552530. PMID: 34221234
7. The type II isoform of cGMP-dependent protein kinase is dimeric and possesses regulatory and catalytic properties distinct from the type I isoforms.  |  Gamm, DM., et al. 1995. J Biol Chem. 270: 27380-8. PMID: 7593002
8. Cloning and expression of a novel cyclic GMP-dependent protein kinase from mouse brain.  |  Uhler, MD. 1993. J Biol Chem. 268: 13586-91. PMID: 8514791
9. Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.  |  Orstavik, S., et al. 1996. Biochem Biophys Res Commun. 220: 759-65. PMID: 8607838
10. N-terminal myristoylation is required for membrane localization of cGMP-dependent protein kinase type II.  |  Vaandrager, AB., et al. 1996. J Biol Chem. 271: 7025-9. PMID: 8636133
11. Signalling by cGMP-dependent protein kinases.  |  Vaandrager, AB. and de Jonge, HR. Mol Cell Biochem. 157: 23-30. PMID: 8739225
12. Role of cGMP-kinase II in the control of renin secretion and renin expression.  |  Wagner, C., et al. 1998. J Clin Invest. 102: 1576-82. PMID: 9788971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cGKII Antibody (D-3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-cGKII Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cGKII Antibody (D-3) Specifications

Alternate Product Name: cGKII

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-120

Epitope Target: cGKII

Epitope Species: human

Product Reactivity/Detection: cGKII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for cGKII Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin