Cdc27 Antibody (B-8): sc-376867
- Cdc27 Antibody (B-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of Cdc27 of human origin
- recommended for detection of Cdc27 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- See Cdc27 (AF3.1): sc-9972 for Cdc27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdc27 Antibody (B-8). This work is in progress.
Cdc27 Antibody (B-8) is a mouse monoclonal IgG1 light chain antibody that detects Cdc27 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc27 plays a crucial role in the regulation of the cell cycle, particularly as a component of the anaphase-promoting complex (APC), which is essential for the timely degradation of cyclins and other cell cycle regulators. This degradation is vital for the transition from metaphase to anaphase during cell division, ensuring that cells do not prematurely enter the next phase of the cycle. The proper functioning of Cdc27 is critical for maintaining genomic stability, as dysregulation can lead to uncontrolled cell proliferation and is often implicated in various cancers. Anti-Cdc27 antibody (B-8) provides a reliable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cell division, making this resource invaluable for advancing our understanding of cellular processes and their implications in disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc27 Antibody (B-8) References:
- The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. | Bermudez, VP., et al. 2002. J Biol Chem. 277: 36853-62. PMID: 12124382
- Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27. | Gray, FC., et al. 2004. BMC Mol Biol. 5: 21. PMID: 15579205
- Molecular structure of the N-terminal domain of the APC/C subunit Cdc27 reveals a homo-dimeric tetratricopeptide repeat architecture. | Zhang, Z., et al. 2010. J Mol Biol. 397: 1316-28. PMID: 20206185
- C/EBP{delta} targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. | Pawar, SA., et al. 2010. Proc Natl Acad Sci U S A. 107: 9210-5. PMID: 20439707
- Anaphase-promoting complex/cyclosome protein Cdc27 is a target for curcumin-induced cell cycle arrest and apoptosis. | Lee, SJ. and Langhans, SA. 2012. BMC Cancer. 12: 44. PMID: 22280307
- mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27. | Feng, Z., et al. 2017. Oncotarget. 8: 6304-6318. PMID: 27974673
- Prostaglandin E2 Inhibition of IL-27 Production in Murine Dendritic Cells: A Novel Mechanism That Involves IRF1. | Hooper, KM., et al. 2017. J Immunol. 198: 1521-1530. PMID: 28062696
- Circular RNA circSLC8A1 inhibits the proliferation and invasion of glioma cells through targeting the miR-214-5p/CDC27 axis. | Zhu, J., et al. 2022. Metab Brain Dis. 37: 1015-1023. PMID: 35098413
- Circ_0000775 promotes the migration, invasion and EMT of hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27. | Zhao, C., et al. 2022. Pathol Res Pract. 235: 153908. PMID: 35561648
- Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3. | Ho, PP., et al. 1995. Somat Cell Mol Genet. 21: 351-5. PMID: 8619132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc27 Antibody (B-8) | sc-376867 | 200 µg/ml | $322.00 | |||
Cdc27 (B-8) Neutralizing Peptide | sc-376867 P | 100 µg/0.5 ml | $69.00 |