Cdc26 Antibody (C-4): sc-377144
- Cdc26 Antibody (C-4) is a mouse monoclonal IgG1 κ Cdc26 antibody provided at 200 µg/ml
- raised against amino acids 1-85 representing full length Cdc26 of human origin
- Cdc26 Antibody (C-4) is recommended for detection of Cdc26 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cdc26 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc26 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc26 Antibody (C-4) (see ordering information below).
Cdc26 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc26 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Cdc26 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc26 plays a crucial role in regulating the anaphase-promoting complex (APC), which is essential for proper cell cycle progression. Specifically, Cdc26 helps stabilize APC, a multi-subunit E3 ubiquitin ligase that marks target proteins for degradation, ensuring DNA replication occurs only once per cell cycle. This regulation maintains genomic stability by preventing DNA re-replication, which can lead to aneuploidy and other cellular abnormalities. Cdc26′s interaction with other APC components, such as Cdc20 and Cdc23, highlights its importance in the transition from metaphase to anaphase, ensuring accurate sister chromatid segregation. Using Cdc26 (C-4) monoclonal antibody in research can provide valuable insights into cell cycle regulation mechanisms and their implications in various diseases, including cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc26 Antibody (C-4) References:
- Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. | Irniger, S., et al. 1995. Cell. 81: 269-78. PMID: 7736579
- Starting to cycle: G1 controls regulating cell division in budding yeast. | Sherlock, G. and Rosamond, J. 1993. J Gen Microbiol. 139: 2531-41. PMID: 8277239
- The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle. | Heichman, KA. and Roberts, JM. 1996. Cell. 85: 39-48. PMID: 8620535
- Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. | Zachariae, W., et al. 1996. Science. 274: 1201-4. PMID: 8895471
- A novel role for Cdc5p in DNA replication. | Hardy, CF. and Pautz, A. 1996. Mol Cell Biol. 16: 6775-82. PMID: 8943332
- Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities. | Levine, K., et al. 1996. Mol Cell Biol. 16: 6794-803. PMID: 8943334
- Cdc20, a beta-transducin homologue, links RAD9-mediated G2/M checkpoint control to mitosis in Saccharomyces cerevisiae. | Lim, HH. and Surana, U. 1996. Mol Gen Genet. 253: 138-48. PMID: 9003297
- The anaphase-promoting complex is required in G1 arrested yeast cells to inhibit B-type cyclin accumulation and to prevent uncontrolled entry into S-phase. | Irniger, S. and Nasmyth, K. 1997. J Cell Sci. 110 (Pt 13): 1523-31. PMID: 9224769
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc26 Antibody (C-4) | sc-377144 | 200 µg/ml | $322.00 | |||
Cdc26 Antibody (C-4): m-IgG Fc BP-HRP Bundle | sc-527582 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc26 Antibody (C-4): m-IgG1 BP-HRP Bundle | sc-532955 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cdc26 Antibody (C-4) AC | sc-377144 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc26 Antibody (C-4) HRP | sc-377144 HRP | 200 µg/ml | $322.00 | |||
Cdc26 Antibody (C-4) FITC | sc-377144 FITC | 200 µg/ml | $336.00 | |||
Cdc26 Antibody (C-4) PE | sc-377144 PE | 200 µg/ml | $349.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 488 | sc-377144 AF488 | 200 µg/ml | $364.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 546 | sc-377144 AF546 | 200 µg/ml | $364.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 594 | sc-377144 AF594 | 200 µg/ml | $364.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 647 | sc-377144 AF647 | 200 µg/ml | $364.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 680 | sc-377144 AF680 | 200 µg/ml | $364.00 | |||
Cdc26 Antibody (C-4) Alexa Fluor® 790 | sc-377144 AF790 | 200 µg/ml | $364.00 |