Anti-Cdc11 Antibody (C-9) is a mouse monoclonal IgG2a (kappa light chain) Cdc11 antibody provided at 200 µg/ml
raised against amino acids 1-415 of Cdc11 of Saccharomyces cerevisiae origin
Cdc11 Antibody (C-9) is recommended for detection of Cdc11 of y origin by WB, IP, IF and ELISA
Anti-Cdc11 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc11 (C-9): sc-166271.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Cdc11 Antibody (C-9) is a high quality monoclonal Cdc11 antibody (also designated Cdc11 antibody) suitable for the detection of the Cdc11 protein of Saccharomyces cerevisiae origin. Cdc11 Antibody (C-9) is available as both the non-conjugated anti-Cdc11 antibody form, as well as multiple conjugated forms of anti-Cdc11 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Extracellular pheromones bind to cell surface receptors and stimulate the activation of the kinase Ste20. This leads to the activation of the MAPKKK Ste11 and the subsequent members of this MAP kinase cascade, Ste7, Fus3 (also called Dac2) and Kss1. These MAP kinases activate Ste12 and Far1, which effect transcriptional and morphological changes necessary for mating. Cdc42, a small GTP-binding protein, is thought to activate Ste20. Cdc42 also plays a role in the polarization of budding. Cla4, a homolog of Ste20, interacts with Cdc42 and is also involved in budding and cytokinesis. Cdc11 is also required for cytokinesis and is present at the bud neck during cell division. The kinase Elm1 regulates morphologic differentiation and is involved in controlling pseudohyphal growth (8,9).
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
For Western Blot, is it recommended to use denatured or non-denatured conditions with Cdc11 (C-9): sc-166271 monoclonal antibody?
Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 4 out of
Nice clean single bandA single clean band against total yeast cell (S. cerevisiae) lysate prepared by TCA precipitation. However, band was quite larger than the expected size of Cdc11 (47 kDa). I did not have a negative control strain to confirm band identity.
Date published: 2018-02-12
Rated 5 out of
Positive signal via western blot analysisPositive signal via western blot analysis of yeast recombinant Cdc11 fusion protein -SCBT QC