CD32 Antibody (KB61): sc-20054
- CD32 Antibody (KB61) is a mouse monoclonal IgG1, cited in 3 publications, provided at 200 µg/ml
- raised against hairy cell leukemia
- recommended for detection of CD32 of human origin by IF and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See CD32 (AT10): sc-13527 for CD32 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD32 Antibody (KB61). These reagents are now offered in bundles with CD32 Antibody (KB61) (see ordering information below).
CD32 Antibody (KB61) is a mouse monoclonal IgG1 antibody that detects CD32 in human samples through immunofluorescence (IF) and flow cytometry (FCM) applications. CD32, also known as Fc gamma RII, serves as a low-affinity receptor for the Fc fragment of aggregated immunoglobulin G (IgG), playing a crucial role in the immune response by mediating the clearance of immune complexes through macrophages. This function is vital for maintaining immune homeostasis and preventing autoimmune diseases, as CD32 helps regulate the levels of circulating antibodies. CD32 is expressed in various cell types, including monocytes, placental trophoblasts, and endothelial cells, with specific isoforms present on B lymphocytes and neutrophils, which underscores CD32′s importance in both innate and adaptive immunity. CD32 exists in several isoforms produced by alternative splicing of three distinct genes (A, B, and C), designated as FcγRIIA, FcγRIIB1, FcγRIIB3, and FcγRIIC, each contributing to the nuanced regulation of immune responses. Anti-CD32 antibody (KB61) is an essential tool for researchers studying immune regulation and the mechanisms underlying antibody production, providing insights into the complex interactions within the immune system.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD32 Antibody (KB61) References:
- Regulatory roles for FcgammaRIII (CD16) and FcgammaRII (CD32) in the development of T- and B-lineage lymphoid cells. | Lynch, RG. 2000. J Leukoc Biol. 67: 279-84. PMID: 10733088
- CD32-Expressing CD4 T Cells Are Phenotypically Diverse and Can Contain Proviral HIV DNA. | Martin, GE., et al. 2018. Front Immunol. 9: 928. PMID: 29780387
- CD32+ and PD-1+ Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals. | Noto, A., et al. 2018. J Virol. 92: PMID: 29976671
- CD32-RNA Co-localizes with HIV-RNA in CD3+ Cells Found within Gut Tissues from Viremic and ART-Suppressed Individuals. | Vásquez, JJ., et al. 2019. Pathog Immun. 4: 147-160. PMID: 31139759
- CD32+CD4+ T Cells Are Highly Enriched for HIV DNA and Can Support Transcriptional Latency. | Darcis, G., et al. 2020. Cell Rep. 30: 2284-2296.e3. PMID: 32075737
- CD32+CD4+ memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice. | Adams, P., et al. 2021. iScience. 24: 101881. PMID: 33364576
- CD32+CD4+ T Cells Sharing B Cell Properties Increase With Simian Immunodeficiency Virus Replication in Lymphoid Tissues. | Huot, N., et al. 2021. Front Immunol. 12: 695148. PMID: 34220857
- Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32. | Yin, J., et al. 2022. Cell Death Dis. 13: 567. PMID: 35739102
- CD32 Expression by CD4+ T and CD8+ T Lymphocytes Is Increased in Patients with Chronic Hepatitis B Virus Infection. | Yao, CY., et al. 2023. Viral Immunol. 36: 351-359. PMID: 37289774
- CD32 (FcγRIIB) expression is low on CD21low B cells from systemic sclerosis patients with digital ulcers, interstitial lung disease, and anti-topoisomerase I autoantibodies. | Kourkouni, E., et al. 2024. Clin Immunol. 262: 110195. PMID: 38494058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD32 Antibody (KB61) | sc-20054 | 200 µg/ml | $316.00 | |||
CD32 Antibody (KB61): m-IgG Fc BP-HRP Bundle | sc-536646 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD32 Antibody (KB61): m-IgGκ BP-HRP Bundle | sc-533804 | 200 µg Ab; 40 µg BP | $354.00 | |||
CD32 Antibody (KB61): m-IgG1 BP-HRP Bundle | sc-544730 | 200 µg Ab; 20 µg BP | $354.00 | |||
CD32 Antibody (KB61) FITC | sc-20054 FITC | 200 µg/ml | $330.00 | |||
CD32 Antibody (KB61) PE | sc-20054 PE | 200 µg/ml | $343.00 |