CD32 Antibody (AT10): sc-13527
- CD32 Antibody (AT10) is a mouse monoclonal IgG1 κ CD32 antibody, cited in 11 publications, provided at 200 µg/ml
- raised against the extracellular domain of CD32 of human origin
- CD32 Antibody (AT10) is recommended for detection of CD32 of human origin by IP, IF and FCM
- Anti-CD32 Antibody (AT10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD32 Antibody (AT10). These reagents are now offered in bundles with CD32 Antibody (AT10) (see ordering information below).
CD32 Antibody (AT10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the CD32 protein of human origin by immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD32 (AT10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. CD32, also known as Fc gamma RII, serves as a low-affinity receptor for the Fc fragment of aggregated immunoglobulin G (IgG), playing a crucial role in the immune response by mediating the clearance of immune complexes through macrophages. This function is vital for maintaining homeostasis and preventing autoimmune reactions, as the binding of IgG to CD32 delivers inhibitory signals to B cells, thereby regulating antibody production. CD32 exists in several isoforms generated by alternative splicing of three distinct genes (A, B, and C), which are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC. These isoforms are expressed on various immune cells, including monocytes, placental trophoblasts, endothelial cells, and B lymphocytes, with specific isoforms found on neutrophils, highlighting CD32′s diverse roles in immune regulation and response.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD32 Antibody (AT10) References:
- Characterization and crystallization of soluble human Fc gamma receptor II (CD32) isoforms produced in insect cells. | Sondermann, P., et al. 1999. Biochemistry. 38: 8469-77. PMID: 10387093
- Functional CD32 molecules on human NK cells. | Morel, PA., et al. 1999. Leuk Lymphoma. 35: 47-56. PMID: 10512162
- Fc receptors. | Ravetch, JV. and Kinet, JP. 1991. Annu Rev Immunol. 9: 457-92. PMID: 1910686
- Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium. | Stuart, SG., et al. 1989. EMBO J. 8: 3657-66. PMID: 2531080
- Binding characteristics of dimeric IgG subclass complexes to human neutrophils. | Huizinga, TW., et al. 1989. J Immunol. 142: 2359-64. PMID: 2784461
- CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir. | Badia, R., et al. 2018. Nat Commun. 9: 2739. PMID: 30013105
- The role of CD32 during HIV-1 infection. | Bertagnolli, LN., et al. 2018. Nature. 561: E17-E19. PMID: 30232425
- The Human FcγRII (CD32) Family of Leukocyte FcR in Health and Disease. | Anania, JC., et al. 2019. Front Immunol. 10: 464. PMID: 30941127
- Direct CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment. | Sconocchia, G., et al. 2022. Life Sci Alliance. 5: PMID: 36241426
- Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells. | Albanese, M., et al. 2024. Cell Rep Med. 5: 101483. PMID: 38579727
- CD32 captures committed haemogenic endothelial cells during human embryonic development. | Scarfò, R., et al. 2024. Nat Cell Biol. 26: 719-730. PMID: 38594587
- Crosslinking of surface immunoglobulin and Fc receptors on B lymphocytes inhibits stimulation of inositol phospholipid breakdown via the antigen receptors. | Bijsterbosch, MK. and Klaus, GG. 1985. J Exp Med. 162: 1825-36. PMID: 3877778
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD32 Antibody (AT10) | sc-13527 | 200 µg/ml | $316.00 | |||
CD32 Antibody (AT10): m-IgG Fc BP-HRP Bundle | sc-526553 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD32 Antibody (AT10): m-IgG1 BP-HRP Bundle | sc-531926 | 200 µg Ab; 20 µg BP | $354.00 | |||
CD32 Antibody (AT10) AC | sc-13527 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD32 Antibody (AT10) HRP | sc-13527 HRP | 200 µg/ml | $316.00 | |||
CD32 Antibody (AT10) FITC | sc-13527 FITC | 200 µg/ml | $330.00 | |||
CD32 Antibody (AT10) PE | sc-13527 PE | 200 µg/ml | $343.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 488 | sc-13527 AF488 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 546 | sc-13527 AF546 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 594 | sc-13527 AF594 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 647 | sc-13527 AF647 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 680 | sc-13527 AF680 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) Alexa Fluor® 790 | sc-13527 AF790 | 200 µg/ml | $357.00 | |||
CD32 Antibody (AT10) B | sc-13527 B | 200 µg/ml | $320.00 |