CD3-γ Antibody (E-2): sc-55563

CD3-γ Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects CD3-γ in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD3-γ is a crucial component of the T cell receptor (TCR) complex, playing a vital role in T cell activation and signaling. Located on the surface of T cells, CD3-γ forms part of a multisubunit complex that includes other invariant polypeptide chains, which together facilitate the recognition of foreign antigens. This recognition triggers a cascade of intracellular signals that lead to T cell activation, essential for the adaptive immune response. The structure of CD3-γ, which contains a single immunoreceptor tyrosine-based activation motif (ITAM), is significant because CD3-γ allows for the recruitment of protein kinases like ZAP-70 and Syk, which are critical for propagating the activation signal. The phosphorylation of the ITAMs creates docking sites for these kinases, enhancing their activity and ensuring a robust immune response. Anti-CD3-γ antibody (E-2) is an invaluable tool for researchers studying T cell biology and the mechanisms underlying immune responses.

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CD3-γ Antibody (E-2) References:

1. The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation.  |  von Essen, M., et al. 2002. J Immunol. 168: 4519-23. PMID: 11970997
2. CD3 and CD2 antigen-mediated CD3 gamma-chain phosphorylation in permeabilized human T cells. Regulation by cytosolic phosphatases.  |  Alexander, DR., et al. 1992. Biochem J. 288 (Pt 1): 69-77. PMID: 1359883
3. Expression of cytoplasmic CD3 epsilon proteins in activated human adult natural killer (NK) cells and CD3 gamma, delta, epsilon complexes in fetal NK cells. Implications for the relationship of NK and T lymphocytes.  |  Lanier, LL., et al. 1992. J Immunol. 149: 1876-80. PMID: 1387664
4. Human T cell leukemia virus type I prevents cell surface expression of the T cell receptor through down-regulation of the CD3-gamma, -delta, -epsilon, and -zeta genes.  |  de Waal Malefyt, R., et al. 1990. J Immunol. 145: 2297-303. PMID: 2144550
5. Characterization of human peripheral lymphocytes expressing the CD3-gamma/delta complex with anti-receptor monoclonal antibodies.  |  Faure, F., et al. 1988. J Immunol. 141: 3357-60. PMID: 3263423
6. CD3 gamma contains a phosphoserine-dependent di-leucine motif involved in down-regulation of the T cell receptor.  |  Dietrich, J., et al. 1994. EMBO J. 13: 2156-66. PMID: 8187769
7. Role of CD3 gamma in T cell receptor assembly.  |  Dietrich, J., et al. 1996. J Cell Biol. 132: 299-310. PMID: 8636209
8. Herpes virus saimiri transformation of T cells in CD3 gamma immunodeficiency: phenotypic and functional characterization.  |  Rodríguez-Gallego, C., et al. 1996. J Immunol Methods. 198: 177-86. PMID: 8946013
9. An amphibian CD3 homologue of the mammalian CD3 gamma and delta genes.  |  Dzialo, RC. and Cooper, MD. 1997. Eur J Immunol. 27: 1640-7. PMID: 9247572
10. Signaling through a CD3 gamma-deficient TCR/CD3 complex in immortalized mature CD4+ and CD8+ T lymphocytes.  |  Pacheco-Castro, A., et al. 1998. J Immunol. 161: 3152-60. PMID: 9743383

The preferred CD3-δ antibody is CD3-δ Antibody (F-1): sc-137137.