Date published: 2026-4-5

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CD14 Antibody (H-4): sc-515785

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Datasheets
  • CD14 Antibody (H-4) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 54-73 near the N-terminus of CD14 of rat origin
  • Anti-CD14 Antibody (H-4) is recommended for detection of CD14 of mouse and rat origin by WB, IP, IF and ELISA
  • Anti-CD14 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD14 Antibody (H-4) for WB applications. These reagents are now offered in bundles with CD14 Antibody (H-4) (see ordering information below).
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CD14 Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that specifically detects CD14 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD14, also known as monocyte differentiation antigen CD14 or myeloid cell-specific leucine-rich glycoprotein, is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of monocytes, macrophages, and neutrophils. CD14 plays a critical role in the innate immune system by acting as a co-receptor for the detection of bacterial lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria. Upon binding LPS, CD14 facilitates the activation of Toll-like receptor 4 (TLR4) signaling pathways, leading to the production of pro-inflammatory cytokines and mediators essential for initiating immune responses against infections. CD14 monoclonal antibody (H-4) recognizes a specific epitope mapping between amino acids 54-73 near the N-terminus of CD14. Anti-CD14 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, making CD14 (H-4) antibody versatile for a wide range of immunological assays and research applications involving CD14-related signaling pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD14 Antibody (H-4)

sc-515785
200 µg/ml
$322.00

CD14 Antibody (H-4): m-IgG Fc BP-HRP Bundle

sc-531513
200 µg Ab; 10 µg BP
$361.00

CD14 Antibody (H-4): m-IgGκ BP-HRP Bundle

sc-525215
200 µg Ab, 40 µg BP
$361.00

CD14 Antibody (H-4): m-IgG2a BP-HRP Bundle

sc-547806
200 µg Ab; 10 µg BP
$361.00

CD14 Antibody (H-4) AC

sc-515785 AC
500 µg/ml, 25% agarose
$424.00

CD14 Antibody (H-4) HRP

sc-515785 HRP
200 µg/ml
$322.00

CD14 Antibody (H-4) FITC

sc-515785 FITC
200 µg/ml
$336.00

CD14 Antibody (H-4) PE

sc-515785 PE
200 µg/ml
$349.00

CD14 Antibody (H-4) Alexa Fluor® 488

sc-515785 AF488
200 µg/ml
$364.00

CD14 Antibody (H-4) Alexa Fluor® 546

sc-515785 AF546
200 µg/ml
$364.00

CD14 Antibody (H-4) Alexa Fluor® 594

sc-515785 AF594
200 µg/ml
$364.00

CD14 Antibody (H-4) Alexa Fluor® 647

sc-515785 AF647
200 µg/ml
$364.00

CD14 Antibody (H-4) Alexa Fluor® 680

sc-515785 AF680
200 µg/ml
$364.00

CD14 Antibody (H-4) Alexa Fluor® 790

sc-515785 AF790
200 µg/ml
$364.00

hi there, is this antibody suitable for neutralization of CD14 in murine cells? thanks

Asked by: Tewfikhamidi
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2023-06-14

Is H-4 a function blocking antibody for rat CD14 and is it formulated in amounts and in buffers appropriate for in vivo studies (i.e. no azide, no carrier protein)?

Asked by: DTCJ
Thank you for your question. We have not tested this antibody for any functional blocking activity, nor are we aware of any publications citing use of the antibody for any blocking function. If you wish to test the antibody yourself for this application, please contact our Special Orders Department for information about ordering it without sodium azide or carrier protein. Please call us toll-free at (800) 457-3801 option 2, or email specialorders@scbt.com.
Answered by: Technical Support
Date published: 2019-07-16

How much sample do you recommend loading on the gel for Western blot detection using CD14 (H-4): sc-515785?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_3, d_27, h_8CST
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Rated 5 out of 5 by from works very wellThis antibody works very well by WB.We tried different conditons and optimized the loading amount and tried different dilution 1:100 to 1:800 all tried.The optimiezd dilution 1:300 is the best.
Date published: 2018-05-26
Rated 3 out of 5 by from Dr.This antibody has two more non-special bands in my blot, when I detected it in the MLE-12 cells
Date published: 2017-10-02
Rated 5 out of 5 by from Identifying and removing macrophageThis antibody is really good for identifying and removing macrophage as contaminating cells in isolates in conjugation with dynabeads magnetic beads. Therefore this is a must have item, will definitely be stocking up!
Date published: 2017-09-14
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CD14 Antibody (H-4) is rated 4.3 out of 5 by 3.
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