CD14 Antibody (UCH-M1) recommended for detection of CD14 monocyte specific cell surface protein of human origin by WB, IP, IF, IHC(P) and FCM

CD14 Antibody (UCH-M1): sc-1182

CD14 Antibody (UCH-M1) is rated 4.0 out of 5 by 12.
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Datasheets

    • CD14 Antibody (UCH-M1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against human thymocytes/Sezary T cells
    • CD14 Antibody (UCH-M1) is recommended for detection of CD14 monocyte specific cell surface protein of human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD14 Antibody (UCH-M1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (UCH-M1): sc-1182.

What negative control should be used when using the conjugated version of CD14 (UCH-M1): sc-1182 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-1182 PE, then you would want to use normal mouse IgG2a PE, sc-2867.
Answered by: Technical Support 9
Date published: 2017-01-11

What secondary is best to use with CD14 (UCH-M1): sc-1182 monoclonal antibody?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with CD14 (UCH-M1): sc-1182, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 4 out of 5 by from good antibody for IF I used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by from Outstanding Great western blot data; strong signal, lowbackground; also superb FCM and IHC results
Date published: 2017-01-28
Rated 5 out of 5 by from Great, versatile antibody The CD14 Antibody (UCH-M1): sc-1182 works well for a variety of applications including WB, IF, IHC(P) and FCM. This antibody has also been cited over 30 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from works great for IF Used it at 1:50-1:100 dilution on PBMCs for 1hr at RT. Worked great, positive cells very bright with no non-specific binding.
Date published: 2016-11-07
Rated 1 out of 5 by from tested in ASTMA tested in ASTMA-23, and Lymph TMA tissue for IHC. Negative @1:25; Not a Good Antibody for IHC
Date published: 2016-06-13
Rated 4 out of 5 by from Various publishable data in FCM using human Various publishable data in FCM using human peripheal blood, THP-1 and PBMC cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Various publishable data in WB using human Various publishable data in WB using human LPS and PGN stimulated uroepithelial cell lines T24, 5637, UM-UC-3, and HT1197, transf. K562 and Human oviduct cell lines. -SCBT Publication Review
Date published: 2015-03-01
Rated 4 out of 5 by from Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded normal human lymph node. -SCBT QC
Date published: 2015-02-24
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