CD14 Antibody (H-4) recommended for detection of CD14 of mouse and rat origin by WB, IP, IF and ELISA

CD14 Antibody (H-4): sc-515785

CD14 Antibody (H-4) is rated 4.3 out of 5 by 3.
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Datasheets

    • CD14 Antibody (H-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 54-73 near the N-terminus of CD14 of rat origin
    • Anti-CD14 Antibody (H-4) is recommended for detection of CD14 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-CD14 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (H-4): sc-515785.

Is H-4 a function blocking antibody for rat CD14 and is it formulated in amounts and in buffers appropriate for in vivo studies (i.e. no azide, no carrier protein)?

Asked by: DTCJ
Thank you for your question. We have not tested this antibody for any functional blocking activity, nor are we aware of any publications citing use of the antibody for any blocking function. If you wish to test the antibody yourself for this application, please contact our Special Orders Department for information about ordering it without sodium azide or carrier protein. Please call us toll-free at (800) 457-3801 option 2, or email [email protected]
Answered by: Technical Support
Date published: 2019-07-16

How much sample do you recommend loading on the gel for Western blot detection using CD14 (H-4): sc-515785?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from works very well This antibody works very well by WB.We tried different conditons and optimized the loading amount and tried different dilution 1:100 to 1:800 all tried.The optimiezd dilution 1:300 is the best.
Date published: 2018-05-26
Rated 3 out of 5 by from Dr. This antibody has two more non-special bands in my blot, when I detected it in the MLE-12 cells
Date published: 2017-10-02
Rated 5 out of 5 by from Identifying and removing macrophage This antibody is really good for identifying and removing macrophage as contaminating cells in isolates in conjugation with dynabeads magnetic beads. Therefore this is a must have item, will definitely be stocking up!
Date published: 2017-09-14
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