CD14 Antibody (H-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 54-73 near the N-terminus of CD14 of rat origin
Anti-CD14 Antibody (H-4) is recommended for detection of CD14 of mouse and rat origin by WB, IP, IF and ELISA
Anti-CD14 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (H-4): sc-515785.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Is H-4 a function blocking antibody for rat CD14 and is it formulated in amounts and in buffers appropriate for in vivo studies (i.e. no azide, no carrier protein)?
Asked by: DTCJ
Thank you for your question. We have not tested this antibody for any functional blocking activity, nor are we aware of any publications citing use of the antibody for any blocking function. If you wish to test the antibody yourself for this application, please contact our Special Orders Department for information about ordering it without sodium azide or carrier protein. Please call us toll-free at (800) 457-3801 option 2, or email [email protected]
Answered by: Technical Support
Date published: 2019-07-16
How much sample do you recommend loading on the gel for Western blot detection using CD14 (H-4): sc-515785?
Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
Rated 5 out of
works very wellThis antibody works very well by WB.We tried different conditons and optimized the loading amount and tried different dilution 1:100 to 1:800 all tried.The optimiezd dilution 1:300 is the best.
Date published: 2018-05-26
Rated 3 out of
Dr.This antibody has two more non-special bands in my blot, when I detected it in the MLE-12 cells
Date published: 2017-10-02
Rated 5 out of
Reviewer 1 from
Identifying and removing macrophageThis antibody is really good for identifying and removing macrophage as contaminating cells in isolates in conjugation with dynabeads magnetic beads. Therefore this is a must have item, will definitely be stocking up!
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