CD14 Antibody (BA-8) recommended for detection of CD14 of human origin by FCM

CD14 Antibody (BA-8): sc-7328

CD14 Antibody (BA-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • CD14 Antibody (BA-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against human monocytes
    • recommended for detection of CD14 of human origin by FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD14 (5A3B11B5): sc-58951 for CD14 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD14 (BA-8): sc-7328 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD14 (BA-8): sc-7328 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Worked great for flow with human stem cells Worked great for flow with human stem cells. -SCBT Publication Review
Date published: 2015-06-13
Rated 5 out of 5 by from Western blot publication with human peripheral Western blot publication with human peripheral blood mononuclear cells. -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by from Published good quality flow cytometry data Published good quality flow cytometry data in human THP-1. -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Produced great FCM data for human peripheral Produced great FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-12-17
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