CD14 Antibody (5A3B11B5) recommended for detection of CD14 of human origin by WB, IP, IF and IHC(P)

CD14 Antibody (5A3B11B5): sc-58951

CD14 Antibody (5A3B11B5) is rated 4.5 out of 5 by 11.
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Datasheets

    • Anti-CD14 Antibody (5A3B11B5) is a mouse monoclonal IgG2b (kappa light chain) CD14 antibody provided at 200 µg/ml
    • raised against recombinant CD14 of human origin
    • CD14 Antibody (5A3B11B5) is recommended for detection of CD14 of human origin by WB, IP, IF and IHC(P)
    • Anti-CD14 Antibody (5A3B11B5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD14 (5A3B11B5): sc-58951.

Hello, Does CD14 Antibody (5A3B11B5) Alexa Fluor® 594 have cross reactivity with mouse? Thank you, Ying

Asked by: YingLi
Thank you for your question. This mouse monoclonal antibody has only been validated for human use at this time, and can not be recommended or warrantied for use on mouse samples. If you have any further questions, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-11

Can this antibody be used with the Li-Cor system? What secondary should be used?

Asked by: AbPolly
Thank you for your question. Yes, this antibody can be used with the Li-COR system. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-01-30
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Rated 5 out of 5 by from great antibody I used this antibody on immunohistochemistry of paraffin embedded tissues and worked great.
Date published: 2017-09-19
Rated 4 out of 5 by from Good antibody I used this antibody to test the changes in T cell acute lymphoblastic leukemia cells like jurkat,it is a Good antibody for WB
Date published: 2017-06-21
Rated 4 out of 5 by from UNclear it is raised in human origin,so i failed to get a chear band in rat
Date published: 2017-03-16
Rated 5 out of 5 by from cd14,membrane-bound CD14, which we procured in accordance with the orientation test requirements, was glycosylphosphatidylinositol-anchored protein found on the surface of monocytes and polymorphonuclear leukocytes. In fact there are two forms of CD14, membrane-bound and soluble forms, and we use the former
Date published: 2017-02-06
Rated 5 out of 5 by from Great CD14 monoclonal choice for WB and IHC(P) good cytoplasmic immunoperoxidase staining in formalin fixed, paraffin-embedded human lymph node tissue
Date published: 2017-01-14
Rated 5 out of 5 by from Excellent There was very strong detection of the ~87 kDa target protein in mouse tissues. Products have a good auxiliary effect, worthy of attention.
Date published: 2017-01-10
Rated 5 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-03-24
Rated 4 out of 5 by from Strong bands seen in WB using BJAB Strong bands seen in WB using BJAB, THP-1 and CCRF-CEM whole cell lysates -SCBT QC
Date published: 2015-03-11
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