CBS Antibody (A-2): sc-133208

CBS Antibody (A-2) is a mouse monoclonal IgG2b antibody that detects cystathionine b-synthase (CBS) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CBS is a crucial cytoplasmic protein predominantly expressed in the human liver and pancreas, with lower levels found in the heart and brain, and CBS plays a vital role in the first step of homocysteine transulfuration, catalyzing the conversion of homocysteine into cystathionine using pyridoxal phosphate as a cofactor. The regulation of CBS activity is significantly influenced by allosteric activation from adenosyl-methionine, which is essential for maintaining proper metabolic function. Deficiencies in CBS can lead to homocystinuria, a genetic disorder characterized by a disruption in sulfur amino acid metabolism that can have severe consequences for multiple organ systems. Symptoms of this condition include arteriosclerosis, thrombosis, dislocated optic lenses, cognitive impairments, and skeletal abnormalities, highlighting the importance of CBS in overall health and metabolic balance. Anti-CBS antibody (A-2) is an invaluable tool for researchers studying the implications of CBS in metabolic disorders and potential therapeutic targets.

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CBS Antibody (A-2) References:

1. CBS domains: structure, function, and pathology in human proteins.  |  Ignoul, S. and Eggermont, J. 2005. Am J Physiol Cell Physiol. 289: C1369-78. PMID: 16275737
2. CRY1-CBS binding regulates circadian clock function and metabolism.  |  Cal-Kayitmazbatir, S., et al. 2021. FEBS J. 288: 614-639. PMID: 32383312
3. ERα/ERβ-directed CBS transcription mediates E2β-stimulated hUAEC H2S production.  |  Bai, J., et al. 2023. J Mol Endocrinol. 70: PMID: 36476832
4. Sleep disturbances in progressive supranuclear palsy syndrome (PSPS) and corticobasal syndrome (CBS).  |  Alster, P., et al. 2023. Neurol Neurochir Pol. 57: 229-234. PMID: 36928793
5. Targeting hypoxia-induced CBS expression inhibits breast cancer stem cells through the induction of ferroptosis.  |  Wei, G., et al. 2023. Genes Dis. 10: 2195-2198. PMID: 37554176
6. Activating transcription factor (ATF) 6 upregulates cystathionine β synthetase (CBS) expression and hydrogen sulfide (H2S) synthesis to ameliorate liver metabolic damage.  |  Dong, B., et al. 2023. Eur J Med Res. 28: 540. PMID: 38007457
7. NR0B1 suppresses ferroptosis through upregulation of NRF2/c-JUN-CBS signaling pathway in lung cancer cells.  |  Zhang, XY., et al. 2023. Am J Cancer Res. 13: 5174-5196. PMID: 38058844
8. KLF10/CBS increases the sensitivity of gastric carcinoma cells to methionine restriction by promoting sulfur transfer pathway.  |  Xin, L., et al. 2024. Exp Cell Res. 437: 114007. PMID: 38499142
9. Benserazide, a cystathionine beta-synthase (CBS) inhibitor, potentially enhances the anticancer effects of paclitaxel via inhibiting the S-sulfhydration of SIRT1 and the HIF1-α/VEGF pathway.  |  Zhao, W., et al. 2024. Front Pharmacol. 15: 1404532. PMID: 38828455
10. Miro2 sulfhydration by CBS/H2S promotes human trophoblast invasion and migration via regulating mitochondria dynamics.  |  Feng, H., et al. 2024. Cell Death Dis. 15: 776. PMID: 39461943

The preferred CBS antibody is CBS Antibody (B-4): sc-133154.