cathepsin C Antibody (D-6): sc-74590

Cathepsin C Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects cathepsin C protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-cathepsin C antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cathepsin C, also known as dipeptidyl aminopeptidase I (DPPI), is a tetrameric lysosomal cysteine peptidase that plays a crucial role in intracellular protein degradation and processing of protein precursors, which is vital for cell growth and activation of various proteins, including neuraminidase and platelet factor XIII. Cathepsin C is primarily localized in secretory granule compartment of cytotoxic lymphocytes and myeloid cells, with highest expression levels found in spleen and kidney. Cathepsin C is synthesized as a proenzyme that undergoes rapid processing to form two distinct chains that work together as active enzyme. Cathepsin C requires halide ions, particularly chloride ions, and thiols for complete enzymatic activity, and interacts with other lysosomal cysteine proteinases, such as cathepsin B, H, and L, highlighting importance in various physiological processes.

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cathepsin C Antibody (D-6) References:

1. Location of the binding site for chloride ion activation of cathepsin C.  |  Cigic, B. and Pain, RH. 1999. Eur J Biochem. 264: 944-51. PMID: 10491143
2. Molecular cloning of cDNA for rat cathepsin C. Cathepsin C, a cysteine proteinase with an extremely long propeptide.  |  Ishidoh, K., et al. 1991. J Biol Chem. 266: 16312-7. PMID: 1885565
3. Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.  |  Xiao, Y., et al. 2021. Cancer Cell. 39: 423-437.e7. PMID: 33450198
4. Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.  |  Yu, J., et al. 2024. Redox Biol. 74: 103231. PMID: 38861835
5. Cathepsin C inhibition reduces neutrophil serine protease activity and improves activated neutrophil-mediated disorders.  |  Nishibata, Y., et al. 2024. Nat Commun. 15: 6519. PMID: 39174512
6. Cathepsin C (dipeptidyl peptidase 1) inhibition in adults with bronchiectasis: AIRLEAF, a phase II randomised, double-blind, placebo-controlled, dose-finding study.  |  Chalmers, JD., et al. 2025. Eur Respir J. 65: PMID: 39255990
7. Molecular cloning and sequence analysis of human preprocathepsin C.  |  Paris, A., et al. 1995. FEBS Lett. 369: 326-30. PMID: 7649281
8. Activation of thrombin-inactivated single-chain urokinase-type plasminogen activator by dipeptidyl peptidase I (cathepsin C).  |  Nauland, U. and Rijken, DC. 1994. Eur J Biochem. 223: 497-501. PMID: 8055919
9. Two distinct gene subfamilies within the family of cysteine protease genes.  |  Karrer, KM., et al. 1993. Proc Natl Acad Sci U S A. 90: 3063-7. PMID: 8464925
10. Human dipeptidyl-peptidase I. Gene characterization, localization, and expression.  |  Rao, NV., et al. 1997. J Biol Chem. 272: 10260-5. PMID: 9092576
11. Molecular cloning, chromosomal localization, and expression of murine dipeptidyl peptidase I.  |  Pham, CT., et al. 1997. J Biol Chem. 272: 10695-703. PMID: 9099719
12. A comparison of four cathepsins (B, L, N and S) with collagenolytic activity from rabbit spleen.  |  Maciewicz, RA. and Etherington, DJ. 1988. Biochem J. 256: 433-40. PMID: 3223923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cathepsin C Antibody (D-6) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-cathepsin C Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cathepsin C Antibody (D-6) Specifications

Alternate Product Name: CTSC

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 251-394

Epitope Target: cathepsin C

Epitope Species: human

Product Reactivity/Detection: cathepsin C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for cathepsin C Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin