caspase-9 p35 Antibody (A-9): sc-133109

caspase-9 p35 Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects the p35 subunit and precursor of caspase-9 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-caspase-9 p35 antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. caspase-9 plays a crucial role in the apoptotic pathway, acting as an initiator caspase that activates downstream effector caspases, such as caspase-3 and caspase-7, thereby facilitating programmed cell death. This process is vital for maintaining cellular homeostasis and eliminating damaged or unwanted cells, which is particularly important in the context of cancer and neurodegenerative diseases. The p35 subunit of caspase-9 is essential for activation and regulation, as p35 helps to form the active caspase-9 complex, which is necessary for the cleavage of specific substrates involved in apoptosis. Understanding caspase-9 function and regulation is critical for developing therapeutic strategies aimed at modulating apoptosis in various diseases.

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caspase-9 p35 Antibody (A-9) References:

1. Regulation of the Apaf-1/caspase-9 apoptosome by caspase-3 and XIAP.  |  Zou, H., et al. 2003. J Biol Chem. 278: 8091-8. PMID: 12506111
2. Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
3. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
4. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death.  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
5. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
6. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
7. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
8. The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex.  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742
9. Caspases: the executioners of apoptosis.  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-9 p35 Antibody (A-9) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-9 p35 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-9 p35 Antibody (A-9) Specifications

Alternate Product Name: caspase-9 p35

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 100-270

Epitope Target: caspase-9 p35

Epitope Species: human

Product Reactivity/Detection: p35 subunit and precursor of caspase-9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for caspase-9 p35 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred caspase-9 antibody is caspase-9 Antibody (96.1.23): sc-56076.