caspase-3 Antibody (46): sc-136219

Caspase-3 Antibody (46) is a mouse monoclonal IgG1 antibody designed to detect caspase-3, also known as apopain, SCA-1, Yama, and CPP32. Caspase-3 monoclonal antibody (46) targets the amino acid region 25-145 of mouse caspase-3, effectively recognizing both the p17 caspase-3 subunit and the full-length procaspase-3 in mouse, rat, and human species through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) assays. Caspase-3 is a pivotal aspartate-specific cysteine protease that plays a critical role in the execution phase of apoptosis, the programmed cell death process essential for maintaining cellular homeostasis and eliminating damaged or unwanted cells. During apoptosis, caspase-3 is activated from its inactive precursor form through proteolytic cleavage, resulting in the formation of an active heterodimer composed of p17 and p11 subunits. This active enzyme then cleaves various substrates, including PARP, facilitating the dismantling of cellular components and ensuring the orderly death of the cell. Additionally, caspase-3 is involved in activating other downstream caspases such as caspase-6, -7, and -9, amplifying the apoptotic signal and ensuring the completion of the cell death process. The importance of caspase-3 extends to its high expression levels in vital organs like the lung, spleen, heart, liver, kidney, and immune cells, highlighting its essential role in diverse physiological functions and disease mechanisms. Anti-caspase-3 antibody (46) is an invaluable tool for researchers studying apoptosis, cancer progression, neurodegenerative disorders, and other conditions where programmed cell death is a key factor.

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.