caspase-3 Antibody (4.1.18): sc-65497

caspase-3 Antibody (4.1.18) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect both active caspase-3 and its inactive precursor, procaspase-3, across mouse, rat, and human species. Caspase-3, also known as apopain, SCA-1, Yama, and CPP32, is a critical aspartate-specific cysteine protease within the ICE subfamily of caspases, characterized by well-defined structural domains essential for function. The protein structure consists of a large (p17) and small (p11) subunit, which form an active heterotetramer upon proteolytic cleavage of the inactive precursor. This structural configuration is vital for caspase-3′s role in apoptosis, allowing efficient cleavage of various substrates, leading to orderly disassembly of cellular components during programmed cell death. The precise arrangement of active sites ensures specificity and regulation of proteolytic activity, making caspase-3 a key executor in the apoptotic pathway. Understanding the structural intricacies of caspase-3 is crucial for research in areas such as cancer, neurodegeneration, and immune response, where apoptosis plays a significant role. caspase-3 (4.1.18) monoclonal antibody provides reliable detection and quantification through applications like western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA).

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.