caspase-12 Antibody (1611): sc-21747

caspase-12 Antibody (1611) is a rat monoclonal IgG2a antibody that detects caspase-12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-caspase-12 antibody (1611) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-12, a member of the Ced-3/caspase-1 family of cysteine proteases, plays a crucial role in apoptotic process by mediating the cleavage of specific target proteins essential for cellular survival and homeostasis. Caspase-12 is primarily located in the endoplasmic reticulum, where caspase-12 is involved in initiating apoptosis in response to cellular stress and inflammation. This localization is significant because caspase-12 enables rapid activation of apoptotic pathways in response to endoplasmic reticulum stress, which can lead to cell death if unresolved. Aberrations in caspase-12 function have been implicated in various diseases, including neurodegenerative disorders and cancer, highlighting caspase-12′s importance as a potential therapeutic target. Caspase-12′s ability to interact with other proteins in the apoptotic signaling cascade underscores caspase-12′s role in regulating cell fate decisions, making caspase-12 vital for understanding apoptosis mechanisms and disease implications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-12 Antibody (1611) References:

1. Frameshift mutations at mononucleotide repeats in caspase-5 and other target genes in endometrial and gastrointestinal cancer of the microsatellite mutator phenotype.  |  Schwartz, S., et al. 1999. Cancer Res. 59: 2995-3002. PMID: 10383166
2. Caspase-12 is involved in stretch-induced apoptosis mediated endoplasmic reticulum stress.  |  Zhang, Q., et al. 2016. Apoptosis. 21: 432-42. PMID: 26801321
3. Caspases and their role in inflammation and ischemic neuronal death. Focus on caspase-12.  |  García de la Cadena, S. and Massieu, L. 2016. Apoptosis. 21: 763-77. PMID: 27142195
4. Caspase-12 Is Present During Craniofacial Development and Participates in Regulation of Osteogenic Markers.  |  Vesela, B., et al. 2020. Front Cell Dev Biol. 8: 589136. PMID: 33178702
5. IL-17A exacerbates caspase-12-dependent neuronal apoptosis following ischemia through the Src-PLCγ-calpain pathway.  |  Wang, H., et al. 2024. Exp Neurol. 379: 114863. PMID: 38871070
6. Caspase-12 affects chondrogenesis in mice.  |  Veselá, B., et al. 2025. Ann Anat. 257: 152347. PMID: 39437937
7. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.  |  Munday, NA., et al. 1995. J Biol Chem. 270: 15870-6. PMID: 7797592
8. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
9. Identification of a cysteine protease closely related to interleukin-1 beta-converting enzyme.  |  Faucheu, C., et al. 1996. Eur J Biochem. 236: 207-13. PMID: 8617266
10. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
11. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
12. Characterization of seven murine caspase family members.  |  Van de Craen, M., et al. 1997. FEBS Lett. 403: 61-9. PMID: 9038361

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-12 Antibody (1611) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-12 Antibody (1611) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-12 Antibody (1611) Specifications

Alternate Product Name: caspase-12

Product Clone Name: 1611

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 95-318

Epitope Target: caspase-12

Epitope Species:

Product Reactivity/Detection: caspase-12

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 39 citations in various scientific publications

Available Conjugates/Forms for caspase-12 Antibody (1611): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred caspase-12 antibody is caspase-12 Antibody (H-6): sc-515103.