CARD 9 Antibody (C-2) recommended for detection of CARD 9 of mouse, rat and human origin by WB, IP, IF and ELISA

CARD 9 Antibody (C-2): sc-374007

CARD 9 Antibody (C-2) is rated 5.0 out of 5 by 1.
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Datasheets

    • CARD 9 Antibody (C-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 98-187 mapping within an internal region of CARD 9 of human origin
    • recommended for detection of CARD 9 of mouse, rat and human origin by WB, IP, IF and ELISA

why it produce two bands by western blot

Asked by: kadhim
Thank you for the question. Two binds of different intensities were repeatedly observed with different Card 9 antibodies in various WB experiments using different sample materials. The nature of the less strong band has not been determined, yet. It most likely represents a Card 9 protein form after post-translational modification.
Answered by: Technical Support Europe
Date published: 2018-02-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CARD 9 (C-2): sc-374007?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced positive Western blot data of CARD Produced positive Western blot data of CARD 9 expression in non-transfected and human CARD 9 transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-11-02
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