CapG Antibody (H-9): sc-166428
- CapG Antibody (H-9) is a mouse monoclonal IgG2b κ CapG antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 308-337 at the C-terminus of CapG of human origin
- CapG Antibody (H-9) is recommended for detection of CapG of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
- Anti-CapG Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CapG Antibody (H-9) for WB applications. These reagents are now offered in bundles with CapG Antibody (H-9) (see ordering information below).
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CapG Antibody (H-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects CapG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CapG antibody (H-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CapG, also known as actin-regulatory protein and macrophage-capping protein, plays a crucial role in regulating actin dynamics within cells. CapG is primarily expressed in macrophages and macrophage-like cells, where CapG functions to reversibly cap barbed ends of actin filaments, preventing further polymerization while not severing existing filaments. This regulation is vital for maintaining cellular structure and motility, particularly in immune responses where macrophages need to rapidly change shape and migrate. Additionally, CapG is a calcium-sensitive DNA-binding protein that can localize to both nucleus and cytoplasm, indicating involvement in various cellular processes beyond actin regulation. CapG interactions with actin are essential for proper functioning of nuclear and cytoplasmic structures, highlighting importance in cellular dynamics and immune function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CapG Antibody (H-9) References:
- Cap G, a gelsolin family protein modulating protective effects of unidirectional shear stress. | Pellieux, C., et al. 2003. J Biol Chem. 278: 29136-44. PMID: 12754261
- Molecular cloning of human macrophage capping protein cDNA. A unique member of the gelsolin/villin family expressed primarily in macrophages. | Dabiri, GA., et al. 1992. J Biol Chem. 267: 16545-52. PMID: 1322908
- Increased importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion. | De Corte, V., et al. 2004. J Cell Sci. 117: 5283-92. PMID: 15454578
- Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG. | Watari, A., et al. 2006. Oncogene. 25: 7373-80. PMID: 16767159
- Gain-of-function mutations conferring actin-severing activity to human macrophage cap G. | Southwick, FS. 1995. J Biol Chem. 270: 45-8. PMID: 7814409
- The human actin-regulatory protein cap G: gene structure and chromosome location. | Mishra, VS., et al. 1994. Genomics. 23: 560-5. PMID: 7851883
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CapG Antibody (H-9) | sc-166428 | 200 µg/ml | $322.00 | |||
CapG Antibody (H-9): m-IgGκ BP-HRP Bundle | sc-521605 | 200 µg Ab, 40 µg BP | $361.00 | |||
CapG Antibody (H-9): m-IgG2b BP-HRP Bundle | sc-548938 | 200 µg Ab; 10 µg BP | $361.00 | |||
CapG Antibody (H-9) AC | sc-166428 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CapG Antibody (H-9) HRP | sc-166428 HRP | 200 µg/ml | $322.00 | |||
CapG Antibody (H-9) FITC | sc-166428 FITC | 200 µg/ml | $336.00 | |||
CapG Antibody (H-9) PE | sc-166428 PE | 200 µg/ml | $349.00 | |||
CapG Antibody (H-9) Alexa Fluor® 488 | sc-166428 AF488 | 200 µg/ml | $364.00 | |||
CapG Antibody (H-9) Alexa Fluor® 546 | sc-166428 AF546 | 200 µg/ml | $364.00 | |||
CapG Antibody (H-9) Alexa Fluor® 594 | sc-166428 AF594 | 200 µg/ml | $364.00 | |||
CapG Antibody (H-9) Alexa Fluor® 647 | sc-166428 AF647 | 200 µg/ml | $364.00 | |||
CapG Antibody (H-9) Alexa Fluor® 680 | sc-166428 AF680 | 200 µg/ml | $364.00 | |||
CapG Antibody (H-9) Alexa Fluor® 790 | sc-166428 AF790 | 200 µg/ml | $364.00 | |||
CapG (H-9) Neutralizing Peptide | sc-166428 P | 100 µg/0.5 ml | $69.00 |