Calpain 2 Antibody (F-11) is a mouse monoclonal IgG2b κ, cited in 6 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 2-27 at the N-terminus of Calpain 2 of human origin
Anti-Calpain 2 Antibody (F-11) is recommended for detection of Calpain 2 precursor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
Anti-Calpain 2 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Calpain 2 (F-11): sc-373967.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Calpain 2 Antibody (F-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with Calpain 2 Antibody (F-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-373967 P appropriate for?
Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
Great for IFThe Calpain 2 Antibody (F-11): sc-373967 works very well for immunofluorescence, showing cytoplasmic localized staining pattern.
Date published: 2017-01-20
Rated 5 out of
Published IHC data with rat spinal cordPublished IHC data with rat spinal cord - SCBT Publication Review
Date published: 2015-03-28
Rated 4 out of
Robust bands seen in WB using ARobust bands seen in WB using A-431 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-15
Rated 5 out of
Superb immunoperoxidase cytoplasmic and membraneSuperb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
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