Calpain 2 Antibody (E-10): sc-373966

Calpain 2 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Calpain 2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Calpain 2 antibody (E-10) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Calpain 2, a crucial intracellular calcium-dependent cysteine protease, plays a significant role in the regulation of cellular processes by cleaving cytoskeletal and submembranous proteins, which is essential for cytoskeletal reorganization and muscle protein degradation. Calpain 2 is primarily located in the cytoplasm and is activated by calcium ions, highlighting its importance in cellular signaling pathways and muscle function. Calpain 2 exists as a heterodimer, consisting of a small regulatory subunit and one of three large catalytic subunits, which include Calpain 1, Calpain 2, and Calpain p94. Calpain activity is tightly regulated by calpastatin, which inhibits both proteolytic activity and membrane binding, with calpastatin existing in tissue and erythrocyte types due to alternative splicing and proteolytic processing. Understanding Calpain 2 function and regulation is vital for elucidating its role in various physiological and pathological processes, making Calpain 2 (E-10) antibody an invaluable tool for researchers studying muscle biology and related disorders.

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Calpain 2 Antibody (E-10) References:

1. Molecular diversity of erythrocyte calpastatin.  |  Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
2. Calpain-2 as a therapeutic target for acute neuronal injury.  |  Wang, Y., et al. 2018. Expert Opin Ther Targets. 22: 19-29. PMID: 29168923
3. Calpain-2 specifically cleaves Junctophilin-2 at the same site as Calpain-1 but with less efficacy.  |  Wang, J., et al. 2021. Biochem J. 478: 3539-3553. PMID: 34524407
4. Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.  |  Wang, LH., et al. 2022. J Neurosci. 42: 5102-5114. PMID: 35606145
5. Calpain-2 Inhibitors as Therapy for Traumatic Brain Injury.  |  Baudry, M., et al. 2023. Neurotherapeutics. 20: 1592-1602. PMID: 37474874
6. T-type voltage-gated channels, Na+/Ca2+-exchanger, and calpain-2 promote photoreceptor cell death in inherited retinal degeneration.  |  Yan, J., et al. 2024. Cell Commun Signal. 22: 92. PMID: 38303059
7. Targeting calpain-2-mediated junctophilin-2 cleavage delays heart failure progression following myocardial infarction.  |  Lahiri, SK., et al. 2024. J Mol Cell Cardiol. 194: 85-95. PMID: 38960317
8. Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin.  |  Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
9. Molecular diversity of calpastatin in human erythroid cells.  |  Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
10. Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain.  |  Elce, JS., et al. 1997. J Biol Chem. 272: 11268-75. PMID: 9111030
11. Calpains: intact and active?  |  Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623
12. Regulation of the calpain-calpastatin system by membranes (review).  |  Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calpain 2 Antibody (E-10) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Calpain 2 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calpain 2 Antibody (E-10) Specifications

Alternate Product Name: Calpain (calcium-dependent cysteine protease) 2

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-27

Epitope Target: Calpain 2

Epitope Species: human

Product Reactivity/Detection: Calpain 2 precursor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Calpain 2 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Calpain 2 antibody is Calpain 2 Antibody (F-11): sc-373967.