Calnexin Antibody (AF18): sc-23954

Calnexin Antibody (AF18) is a mouse monoclonal IgG1 kappa light chain antibody raised against a human hepatoma cell line, reactive with Calnexin in mouse, rat, and human samples. Calnexin, also known as CNX, IP90, P90, or CANX, is a calcium-binding chaperone protein localized in the endoplasmic reticulum (ER) membrane. As a type I integral membrane protein, Calnexin plays a crucial role in the quality control system of the ER by interacting with newly synthesized N-linked glycoproteins, ensuring their proper folding and assembly. This function is important because it prevents the accumulation of misfolded proteins, which can lead to cellular stress and diseases such as neurodegeneration. Calnexin binds to monoglucosylated glycoproteins, retaining them in the ER until they achieve their correct conformation. By facilitating the maturation of glycoproteins, Calnexin maintains protein homeostasis within the cell. Anti-Calnexin antibody (AF18) is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP), making this antibody a valuable tool for researchers studying protein folding, ER stress, and related cellular processes.

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Calnexin Antibody (AF18) References:

1. Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein.  |  Smith, MJ. and Koch, GL. 1989. EMBO J. 8: 3581-6. PMID: 2583110
2. The Merck Frosst Award Lecture 1994/La conference Merck Frosst 1994. Calnexin: a molecular chaperone with a taste for carbohydrate.  |  Williams, DB. 1995. Biochem Cell Biol. 73: 123-32. PMID: 7576485
3. Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms.  |  Wada, I., et al. 1995. J Biol Chem. 270: 20298-304. PMID: 7657600
4. Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5.  |  Tjoelker, LW., et al. 1994. Biochemistry. 33: 3229-36. PMID: 8136357
5. 2,4,6-Trinitrobenzenesulfonic acid modification of the carboxyl-terminal region (C-domain) of calreticulin.  |  Breier, A. and Michalak, M. 1994. Mol Cell Biochem. 130: 19-28. PMID: 8190118
6. Interaction with newly synthesized and retained proteins in the endoplasmic reticulum suggests a chaperone function for human integral membrane protein IP90 (calnexin).  |  David, V., et al. 1993. J Biol Chem. 268: 9585-92. PMID: 8486646

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calnexin Antibody (AF18) has 186 citations in a variety of scientific publications.

Primary antibodies like Anti-Calnexin Antibody (AF18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calnexin Antibody (AF18) Specifications

Alternate Product Name: CANX

Product Clone Name: AF18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Calnexin

Epitope Species: human

Product Reactivity/Detection: Calnexin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 186 citations in various scientific publications

Available Conjugates/Forms for Calnexin Antibody (AF18): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin