CagA Antibody (A-10): sc-28368
- CagA Antibody (A-10) is a mouse monoclonal IgG2a κ CagA antibody, cited in 54 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of CagA of H. pylori origin
- CagA Antibody (A-10) is recommended for detection of CagA of H. pylori origin by WB, IP and ELISA
- Anti-CagA Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CagA Antibody (A-10) for WB applications. These reagents are now offered in bundles with CagA Antibody (A-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
CagA Antibody (A-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects CagA protein of H. pylori origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). CagA Antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CagA, a key virulence factor of Helicobacter pylori, plays a critical role in the pathogenesis of gastric diseases, including ulcers and gastric cancer. Once injected into host cells, CagA undergoes phosphorylation, which is essential for function as phosphorylated CagA interacts with various host cell signaling pathways. This post-translational modification enables CagA to manipulate host cell behavior, leading to changes in cell morphology, actin cytoskeleton rearrangement, and disruption of normal cellular processes. CagA′s ability to induce these alterations is linked to oncogenic potential, making CagA a significant factor in the development of gastric cancer. Understanding CagA′s role and modifications not only sheds light on H. pylori pathogenesis but also highlights potential therapeutic targets for managing H. pylori-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CagA Antibody (A-10) References:
- Mechanisms of Helicobacter pylori infection: bacterial factors. | McGee, DJ. and Mobley, HL. 1999. Curr Top Microbiol Immunol. 241: 155-80. PMID: 10087661
- Helicobacter pylori associated gastric pathology. | Konturek, PC., et al. 1999. J Physiol Pharmacol. 50: 695-710. PMID: 10695552
- Disease-specific Helicobacter pylori virulence factors: the unfulfilled promise. | Graham, DY. and Yamaoka, Y. 2000. Helicobacter. 5 Suppl 1: S3-9; discussion S27-31. PMID: 10828748
- Cellular responses induced after contact with Helicobacter pylori. | Censini, S., et al. 2001. Curr Opin Microbiol. 4: 41-6. PMID: 11173032
- Virulence factors of Helicobacter pylori. | Dundon, WG., et al. 2001. Int J Med Microbiol. 290: 647-58. PMID: 11310443
- Increased risk of developing atrophic gastritis in patients infected with CagA+ Helicobacter pylori. | Sande, N., et al. 2001. Scand J Gastroenterol. 36: 928-33. PMID: 11521982
- Molecular anatomy and pathogenic actions of Helicobacter pylori CagA that underpin gastric carcinogenesis. | Takahashi-Kanemitsu, A., et al. 2020. Cell Mol Immunol. 17: 50-63. PMID: 31804619
- Helicobacter pylori CagA elicits BRCAness to induce genome instability that may underlie bacterial gastric carcinogenesis. | Imai, S., et al. 2021. Cell Host Microbe. 29: 941-958.e10. PMID: 33989515
- CagA+ Helicobacter pylori, Not CagA- Helicobacter pylori, Infection Impairs Endothelial Function Through Exosomes-Mediated ROS Formation. | Xia, X., et al. 2022. Front Cardiovasc Med. 9: 881372. PMID: 35433874
- A review of signal pathway induced by virulent protein CagA of Helicobacter pylori. | Wang, H., et al. 2023. Front Cell Infect Microbiol. 13: 1062803. PMID: 37124036
- Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer. | Peng, Y., et al. 2024. Exp Mol Med. 56: 441-452. PMID: 38383581
- Helicobacter pylori promotes gastric cancer through CagA-mediated mitochondrial cholesterol accumulation by targeting CYP11A1 redistribution. | Zhang, Z., et al. 2024. Int J Biol Sci. 20: 4007-4028. PMID: 39113698
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CagA Antibody (A-10) | sc-28368 | 200 µg/ml | $316.00 | |||
CagA Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-528437 | 200 µg Ab; 10 µg BP | $354.00 | |||
CagA Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-520812 | 200 µg Ab, 40 µg BP | $354.00 | |||
CagA Antibody (A-10): m-IgG2a BP-HRP Bundle | sc-547097 | 200 µg Ab; 10 µg BP | $354.00 | |||
CagA Antibody (A-10) AC | sc-28368 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CagA Antibody (A-10) HRP | sc-28368 HRP | 200 µg/ml | $316.00 | |||
CagA Antibody (A-10) FITC | sc-28368 FITC | 200 µg/ml | $330.00 | |||
CagA Antibody (A-10) PE | sc-28368 PE | 200 µg/ml | $343.00 | |||
CagA Antibody (A-10) Alexa Fluor® 488 | sc-28368 AF488 | 200 µg/ml | $357.00 | |||
CagA Antibody (A-10) Alexa Fluor® 546 | sc-28368 AF546 | 200 µg/ml | $357.00 | |||
CagA Antibody (A-10) Alexa Fluor® 594 | sc-28368 AF594 | 200 µg/ml | $357.00 | |||
CagA Antibody (A-10) Alexa Fluor® 647 | sc-28368 AF647 | 200 µg/ml | $357.00 | |||
CagA Antibody (A-10) Alexa Fluor® 680 | sc-28368 AF680 | 200 µg/ml | $357.00 | |||
CagA Antibody (A-10) Alexa Fluor® 790 | sc-28368 AF790 | 200 µg/ml | $357.00 |