CAD Antibody (G-11): sc-393029
- CAD Antibody (G-11) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 310-338 at the C-terminus of CAD of human origin
- recommended for detection of CAD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CAD Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with CAD Antibody (G-11) (see ordering information below).
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CAD Antibody (G-11) is a mouse monoclonal IgG1 antibody that detects CAD (caspase-activated deoxyribonuclease) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CAD plays a crucial role in the apoptotic process by facilitating DNA degradation, which is essential for the orderly dismantling of cellular components during programmed cell death. CAD (G-11) antibody targets a protein primarily located in the nucleus, where CAD becomes activated following cleavage by caspase-3, which dissociates CAD from its inhibitor, ICAD. Nuclear localization of CAD is significant because CAD allows for targeted degradation of DNA, ensuring efficient execution of the apoptotic process and preventing potential genomic instability from incomplete or improper apoptosis. Anti-CAD antibody (G-11) serves as an invaluable tool for researchers studying apoptosis and its implications in various diseases, including cancer, where dysregulation of this process can lead to uncontrolled cell proliferation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CAD Antibody (G-11) References:
- Induction of apoptosis by penta-O-galloyl-beta-D-glucose through activation of caspase-3 in human leukemia HL-60 cells. | Pan, MH., et al. 1999. Eur J Pharmacol. 381: 171-83. PMID: 10554885
- Selenite negatively regulates caspase-3 through a redox mechanism. | Park, HS., et al. 2000. J Biol Chem. 275: 8487-91. PMID: 10722685
- The antiangiogenic factor 16K PRL induces programmed cell death in endothelial cells by caspase activation. | Martini, JF., et al. 2000. Mol Endocrinol. 14: 1536-49. PMID: 11043570
- Induction of apoptosis by garcinol and curcumin through cytochrome c release and activation of caspases in human leukemia HL-60 cells. | Pan, MH., et al. 2001. J Agric Food Chem. 49: 1464-74. PMID: 11312881
- Induction of apoptosis by hydroxydibenzoylmethane through coordinative modulation of cyclin D3, Bcl-X(L), and Bax, release of cytochrome c, and sequential activation of caspases in human colorectal carcinoma cells. | Pan, MH., et al. 2003. J Agric Food Chem. 51: 3977-84. PMID: 12822933
- Solution structure of a ubiquitin-like domain from tubulin-binding cofactor B. | Lytle, BL., et al. 2004. J Biol Chem. 279: 46787-93. PMID: 15364906
- Molecular mechanisms of (-)-gossypol-induced apoptosis in human prostate cancer cells. | Huang, YW., et al. 2006. Anticancer Res. 26: 1925-33. PMID: 16827126
- Induction of G1 arrest and apoptosis by Scutellaria barbata in the human promyelocytic leukemia HL-60 cell line. | Kim, EK., et al. 2007. Int J Mol Med. 20: 123-8. PMID: 17549398
- Triggering of apoptosis by Puma is determined by the threshold set by prosurvival Bcl-2 family proteins. | Callus, BA., et al. 2008. J Mol Biol. 384: 313-23. PMID: 18835564
- Marein protects against methylglyoxal-induced apoptosis by activating the AMPK pathway in PC12 cells. | Jiang, B., et al. 2016. Free Radic Res. 50: 1173-1187. PMID: 27596733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAD Antibody (G-11) | sc-393029 | 200 µg/ml | $322.00 | |||
CAD Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-538120 | 200 µg Ab; 10 µg BP | $361.00 | |||
CAD Antibody (G-11): m-IgGκ BP-HRP Bundle | sc-535675 | 200 µg Ab; 40 µg BP | $361.00 | |||
CAD Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-545532 | 200 µg Ab; 20 µg BP | $361.00 | |||
CAD (G-11) Neutralizing Peptide | sc-393029 P | 100 µg/0.5 ml | $69.00 |